Cyclin e sc 377100

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Cyclin E (sc-377100) is a lab equipment product from Santa Cruz Biotechnology. It is an antibody that specifically recognizes cyclin E, a protein involved in cell cycle regulation. The core function of this product is to detect and quantify the presence of cyclin E in biological samples.

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Cyclin E (199 citations) SC-377100 (3 citations)

2 protocols using cyclin e sc 377100

Molecular Analysis of 6-Gingerol Effects

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Roswell Park Memorial Institute-1640 (RPMI-1640) medium, penicillin-streptomycin solution, and trypsin-EDTA (0.05%) were all purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). 6-Gingerol (cat no. 23513-14-6) was purchased from TCI (Tokyo Chemical Industry Co., Tokyo, Japan). Fetal bovine serum (FBS) was obtained from Sigma–Aldrich (Merck KGaA, St. Louis, MO, USA). Recombinant human EGF (AF-100-15) was purchased from Peprotech, Inc. (Rocky Hill, NJ, USA). Antibodies specific for CDK4 (sc-23896), BCL-2 (sc-7382), p21 (sc-6246), and cyclin E (sc-377100) together with secondary antibodies (anti-mouse (sc-516102) and anti-rabbit (sc-2357)) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies specific for pEGFR (#3777), EGFR (#4267), pSTAT3 (#9145), STAT3 (#9139), cyclin D1 (#2922), p27 (#3686), p53 (#9282), pCHK1 (#2348), pATM (#5883), pBRCA1 (#9009), pHistone H2A.X (#9718), pCHK2 (#2197), BAX (#2772), Casp 9 (#9502), C-Casp 9 (#9505), cytochrome c (#11940), BCL-xL (#2764), pMDM2 (#3521), MDM2 (#86934), pSrc (#6943), Src (#2109), and GAPDH (#2118) were obtained from Cell Signaling Technology (Beverly, MA, USA). Finally, iNOS antibody (NB300-650) was purchased from Novus Biologicals (Littleton, CO, USA).

Sp N., Kang D.Y., Lee J.M., Bae S.W, & Jang K.J. (2021). Potential Antitumor Effects of 6-Gingerol in p53-Dependent Mitochondrial Apoptosis and Inhibition of Tumor Sphere Formation in Breast Cancer Cells. International Journal of Molecular Sciences, 22(9), 4660.

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Western Blot and Immunoprecipitation Assays

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The western blot (WB) and immunoprecipitation assays were performed as described previously. 37 The primary antibodies against EGFR (#4267S, 1:500), phospho-EGF receptor (p-EGFR) (#3777S, 1:500), EGFR E746-A750del (#2085S, 1:500/WB, 1:100/IP), EGFR L858R (#3197S, 1:500), YAP (#14074, 1:500), p-YAP (#13008, 1:500), MST1 (#14946, 1:500), p-MST1 (#49332, 1:500), LATS1 (#3477, 1:500), p-LATS1 (#9157, 1:500), SIK2 (#6919, 1:500/WB, 1:100/IP), p-Stat5 (#4322, 1:500), p-Gab1 (#12745, 1:500), p-Shc (#2434, 1:500), p-PLCg1 (#2821, 1:500), SAV1 (#1330, 1:500/WB, 1:100/IP), ATP1A1 (#23565, 1:500), Histone H3 (#4499, 1:500), GAPDH (#5174, 1:10000), and phospho-p44/42 MAPK (Erk1/2, #8544, 1:500) were purchased from CST Inc. Primary antibodies against LaminB1 (#ab16048, 1:1000/IB) and tubulin (#ab52866, 1:1000/IB) were purchased from Abcam (Cambridge, Massachusetts) and those against p-ERK 1/2 (sc-81492), CyclinE (sc-377100, 1:200), and CTGF (sc-34772, 1:200) were from Santa Cruz Biotechnology (Santa Cruz, California). Secondary antibodies were purchased from CST Inc. Expression was quantified using densitometry and ImageJ software. Protein complexes obtained by immunoprecipitation were detected and analyzed by Hoogen Biotechnology Company (Shanghai, China) for mass spectrometry.

Rong X., Liang Y., Han Q., Zhao Y., Jiang G., Zhang X., Lin X., Liu Y., Zhang Y., Han X., Zhang M., Luo Y., Li P., Wei L., Yan T, & Wang E. (2019). Molecular Mechanisms of Tyrosine Kinase Inhibitor Resistance Induced by Membranous/Cytoplasmic/Nuclear Translocation of Epidermal Growth Factor Receptor. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 14(10).

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