Ph meter seven multi

The samples were incubated in a water bath at 37 °C for 60 min. After incubation, pH was measured with pH meter Seven Multi (Mettler-Toledo GmbH, Greifensee, Switzerland), four 10 mL repetitions of every sample were put in the setting block, and 200 µL of rennet solution was added. The analysis was set for 60 min, and the results were later retrieved for statistical analysis.

Milk fat, protein, total solids, non-fat solids, and lactose were determined on the Milkoscan FT3 (Foss, Hilleroed, Denmark) according to ISO 9622:2013 [36 ]. Somatic cell count was determined on the Fossomatic Minor (Foss, Hilleroed, Denmark) with the enumeration of somatic cells, fluoro–opto–electronic method according to ISO 13366-3 [37 ]. The total bacterial count (TBC) was determined with the horizontal method for the enumeration of microorganisms—colony counts at 30 °C by the pour plate technique, ISO 4833-1:2013 and ISO 21187:2021 [38 ,39 ] in plate count agar with skim milk (Biolife Italiana S.r.l, Milano, Italy). Milk acidity was measured with pH meter Seven Multi (Mettler-Toledo GmbH, Greifensee, Switzerland) according to the manufacturer’s instructions.