Western blotting analysis was performed as previously reported.42 (link) Antibodies for LC3B (L7543), β-actin (A5441) and asparagine synthetase (A6485) were purchased from Sigma-Aldrich; ATF4 (L0911) was from Santa Cruz Biotechnology (Dallas, TX, USA); p53 (OP43L) was from Calbiochem (San Diego, CA, USA); cleaved PARP (#9541), cleaved CASP3 (#9661), CASP3 (#9662), CHOP (#2895), BECN1 (#4122) and ATG5 (#12994) were from Cell Signaling (Danvers, MA, USA); γH2AX (ab11174) was from Abcam.
γh2ax ab11174
Manufactured by Abcam
Sourced in United States
γH2AX (ab11174) is an antibody that recognizes phosphorylated histone H2AX, a marker of DNA double-strand breaks. It can be used to detect and quantify DNA damage in cells.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved parp 9541, by Cell Signaling Technology (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Cox 4, by Cell Signaling Technology (1 mentions)
Plx4720, by Selleck Chemicals (1 mentions)
Plx4032, by Selleck Chemicals (1 mentions)
Actin a2066, by Merck Group (1 mentions)
Navitoclax, by Selleck Chemicals (1 mentions)
Azd5991, by Selleck Chemicals (1 mentions)
P rb s807 811, by Cell Signaling Technology (1 mentions)
Vinculin, by Cell Signaling Technology (1 mentions)
Lamp1, by Cell Signaling Technology (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Erk2 sc 1647, by Santa Cruz Biotechnology (1 mentions)
Pt308 akt, by Cell Signaling Technology (1 mentions)
Vcam 1, by Cell Signaling Technology (1 mentions)
Trametinib gsk1120212, by Selleck Chemicals (1 mentions)
P pras40 t246, by Cell Signaling Technology (1 mentions)
Buparlisib bkm120, by Selleck Chemicals (1 mentions)
Plx8394, by Selleck Chemicals (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
2 protocols using γh2ax ab11174
1
Western Blotting Analysis of Apoptotic Markers
2
Comprehensive Molecular Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Trametinib (GSK1120212) (#S2673), PLX4720 (#S1152), PLX4032 (#S1267), PLX8394 (#S7965), AZD5991 (#S8643), MK2206 (#S1078), buparlisib (BKM120) (#S2247), and Navitoclax (#S1001) were purchased from Selleck Chemicals, LLC. DRP-1 (#8570S), BAK (#12105S), Bcl-XL (2762S), Caspase 9 (#9502S), Cleaved caspase 3 (#9661S), HSP90 (#4877S), pERK1/2 (#9101S), pRb S807/811 (#9308S), pT308 AKT (#2965S), pPRAS40 T246 (#2640S), VCAM1 (#13662S), Mcl-1 (#4572S), LAMP-1 (#9091T), Vinculin (#4650S), Tubulin (#2148S), COX-IV (#11967S), cytochrome c (#11940S) cleaved PARP (#9541) and GAPDH (#2118S) antibodies were purchased from Cell Signaling Technology. ERK2 (sc-1647) and AIF (#sc-13116) were purchased from Santa Cruz Biotechnology Inc., γH2AX (ab11174) was purchased from Abcam, Actin (A2066) was purchased from Sigma-Aldrich Co., and Bcl-2 (#51-6511GR) was purchased from BD Biosciences. Goat anti-rabbit IgG Alexa FluorTM 488 secondary antibody was purchased from Invitrogen.
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