Tumors were fixed in 4% paraformaldehyde and paraffin embedded. When appropriate, control sections were treated with hyaluronidase (16 U/mL) for 30min, in a humidity chamber at 37 °C. Sections were then stained with either hematoxylin and eosin (H&E), F4/80 (#MCA497GA at 1:100, Biorad, Hercules, CA, USA) and CD206 (Abcam #ab64693 at 1:1000, Cambridge, UK) overnight at 4 °C, or biotinylated HABP (MilliporeSigma #385911 at 1:100), for 1 h at room temperature. Sections were incubated in the appropriate secondary antibodies for 1 h at room temperature in a humidity chamber: Streptavidin AF 488 conjugate (at 1:500, FisherScientific #S11223, Waltham, MA, USA), AF 594 (at 1:400, Invitrogen #A11007), and AF 488 (at 1:400, Fisher Scientific #A21206). Tissues were coverslipped with ProLong Gold Antifade DAPI (Invitrogen, #P36931).
Af 488
Manufactured by Thermo Fisher Scientific
Sourced in United States
AF 488 is a fluorescent dye commonly used in laboratory applications. It has an excitation maximum at 488 nm and an emission maximum at 515 nm. AF 488 is known for its bright fluorescence and photostability.
Automatically generated - may contain errors
2 protocols using af 488
1
Histological Analysis of Tumor Microenvironment
2
VLP Tumor Targeting and Delivery
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CuMVTT were labeled with AF488 (Fisher Scientific) for intratumoral injection with or without formulation with MCT in RAG−/− mice. Twelve days later, intratumoral injections with AF488-CuMVTT or AF488-CuMVTT+MCT were performed. Injected tumors were collected 1 and 5 days post injection, and analysis was performed to detect labeled VLPs.
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