Primary antibodies to the following proteins were used: Erg (ab92513; Abcam), NG2 (AB5320; Merck Millipore), endomucin (sc-65495; Santa Cruz Biotechnology), LYVE-1 (AF2125; R&D Systems), PROX-1 (PRB-238C; Covance), and Cleaved caspase-3 (9664S; Cell Signalling Technology). Secondary antibodies conjugated to Cy3, Cy5, or AlexaFluor 647 were from Jackson ImmunoResearch Laboratories. For immunohistochemistry (IHC), biotinylated antibodies were from Vector Laboratories. Isolectin GS-IB4 conjugated to AlexaFluor 488 (I21411) or AlexaFluor 568 (I21412) (further referred to as IB4) was from Life Technologies. Immu-Mount mounting medium was from Thermo Scientific. AuroVist 15 nm gold nanoparticles were from Nanoprobes Inc. Rapamycin used for therapeutic studies was obtained from Merck Millipore. All chemicals, unless otherwise stated, were from Sigma-Aldrich.
Ng2 ab5320
Manufactured by Merck Group
Sourced in United States
NG2 (AB5320) is a lab equipment product manufactured by Merck Group. It serves as a core functional component for laboratory applications. The detailed specifications and intended use of this product are not available for an unbiased and factual description.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated antibody, by Vector Laboratories (1 mentions)
Immu mount mounting medium, by Thermo Fisher Scientific (1 mentions)
Endomucin sc 65495, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Alexa fluor 647, by Jackson ImmunoResearch (1 mentions)
Gapdh 5174, by Cell Signaling Technology (1 mentions)
Recombinant murine il 1β, by Thermo Fisher Scientific (1 mentions)
2 protocols using ng2 ab5320
1
Immunohistochemical Analysis of Vascular Markers
2
Immunofluorescence and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Monoclonal Claudin-5 antibody (#35–2500) was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Polyclonal GFAP antibody (#NB300–141) and CD11b/c antibody (#NB 110–70466) were purchased from Novus Biologicals, LLC (Centennial, CO, USA). Neuronal nuclei (NeuN #MAB377), synapsin (Syn1 #AB1543), oligodendrocyte transcription factor 2 (Olig2 #MABN50) and Neural/glial antigen 2 (NG2 #AB5320) antibodies were purchased from EMD Millipore (Billerica, MA, USA). Myelin basic protein (MBP #13344), neurofilament-Light chain (NFL #2837), and GAPDH (#5174) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Secondary anti-mouse-HRP and anti-rabbit-HRP antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Secondary Alex Fluor goat anti-rabbit IgG-488 (H + L) (A11034) and Alex Fluor goat anti-mouse-594 (H + L) (A11032) were purchased from Invitrogen Inc. (Thermo Fisher Scientific). Recombinant murine IL-1β (Cat# 211-11B) was purchased from PeproTech Inc (Rocky Hill, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!