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Anti annexin 5 alexa fluor 488 conjugate a13201

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Anti-Annexin V Alexa Fluor 488 conjugate (A13201) is a fluorescently labeled protein that binds to Annexin V. It is used for the detection and quantification of Annexin V, a protein that is involved in various cellular processes.

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Lab products found in correlation

Ab2907, by Abcam (2 mentions) Ab10287, by Abcam (2 mentions) Ab150081, by Abcam (2 mentions) Rabbit polyclonal anti parp 9542, by Cell Signaling Technology (2 mentions) Z vad fmk, by Promega (2 mentions) 7 aad, by Thermo Fisher Scientific (2 mentions) Brefeldin a, by BD (2 mentions)

2 protocols using anti annexin 5 alexa fluor 488 conjugate a13201

1

Synthesis and Characterization of Polyamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were purchased from Sigma-Aldrich unless otherwise noted. Py–Im polyamides were synthesized by microwave-assisted, solid-phase synthesis on Kaiser oxime resin (Novabiochem) according to previously described protocols (Baird & Dervan, 1996 ; Puckett et al. 2012 (link)). Polyamide 5 was synthesized on hydrazine resin (855037, Novabiochem) in the same manner as the other polyamides and cleaved from resin in the same manner after 10 min oxidation of the hydrazine by Cu(II)SO4 in pyridine and DMF. Polyamides were purified by reverse-phase HPLC and lyophilized. EZ-Link NHS-PEG4-biotin (Pierce) was conjugated in 5% Hünig’s base in DMF. Purity and identity of compounds were verified by analytical HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SI Table 2).
Brefeldin A was purchased from BD Biosciences, 7-AAD from eBiosciences and Z-VAD-fmk from Promega. Antibodies purchased from Abcam are: polyclonal rabbit anti-calreticulin (ab2907), polyclonal rabbit anti-ERp57 (ab10287), polyclonal goat anti-rabbit Alexa Fluor 488 (ab150081). Antibodies from Life Technologies are: anti-Annexin V Alexa Fluor 488 conjugate (A13201) and mouse monoclonal anti-actin (AM4302). Antibodies from Cell Signaling Technologies are: rabbit polyclonal anti-PARP (9542) and rabbit monoclonal anti-phospho-H2AX (ser139, 9718).
Kang J.S, & Dervan P.B. (2015). A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma. Quarterly reviews of biophysics, 48(4), 453-464.
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2

Synthesis and Characterization of Polyamides

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were purchased from Sigma-Aldrich unless otherwise noted. Py–Im polyamides were synthesized by microwave-assisted, solid-phase synthesis on Kaiser oxime resin (Novabiochem) according to previously described protocols (Baird & Dervan, 1996 ; Puckett et al. 2012 (link)). Polyamide 5 was synthesized on hydrazine resin (855037, Novabiochem) in the same manner as the other polyamides and cleaved from resin in the same manner after 10 min oxidation of the hydrazine by Cu(II)SO4 in pyridine and DMF. Polyamides were purified by reverse-phase HPLC and lyophilized. EZ-Link NHS-PEG4-biotin (Pierce) was conjugated in 5% Hünig’s base in DMF. Purity and identity of compounds were verified by analytical HPLC and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SI Table 2).
Brefeldin A was purchased from BD Biosciences, 7-AAD from eBiosciences and Z-VAD-fmk from Promega. Antibodies purchased from Abcam are: polyclonal rabbit anti-calreticulin (ab2907), polyclonal rabbit anti-ERp57 (ab10287), polyclonal goat anti-rabbit Alexa Fluor 488 (ab150081). Antibodies from Life Technologies are: anti-Annexin V Alexa Fluor 488 conjugate (A13201) and mouse monoclonal anti-actin (AM4302). Antibodies from Cell Signaling Technologies are: rabbit polyclonal anti-PARP (9542) and rabbit monoclonal anti-phospho-H2AX (ser139, 9718).
Kang J.S, & Dervan P.B. (2015). A sequence-specific DNA binding small molecule triggers the release of immunogenic signals and phagocytosis in a model of B-cell lymphoma. Quarterly reviews of biophysics, 48(4), 453-464.
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