Intrahepatic lymphocytes were extracted by the above method. The following antibodies that bind to surface or intracellular markers of mice lymphocytes were used: FITC anti-mouse CD4 (clone H129.19, Biolegend, San Diego, CA, USA), APC anti-mouse CD25 (clone 3C7, Biolegend), PE anti-mouse FOXP3 (clone MF-14, Biolegend), PE anti-mouse IL-17A (clone TC11–18H10.1, Biolegend). FITC-CD4 and APC-CD25 were used to indicate Treg cell surface markers, and PE-FOXP3 was used to indicate intracellular markers in Treg cells. FITC-CD4 was used to indicate surface markers in Th17 cells, and PE-IL-17A was used to indicate intracellular markers in Th17 cells. Wash cells after flow cytometry antibody staining in the dark and resuspend with 100 µL staining buffer for flow cytometry. The results were captured by a BD FACS Aria III Flow Cytometer (BD Bioscience, San Jose, CA, USA) and analyzed using FlowJo 10.4 software (BD Bioscience, San Jose, CA, USA).
Pe anti mouse foxp3 clone mf 14
Manufactured by BioLegend
Sourced in United States
PE anti-mouse FOXP3 (clone MF-14) is a fluorescently-labeled antibody that specifically binds to the mouse FOXP3 transcription factor. FOXP3 is a key regulator of regulatory T cell (Treg) development and function.
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Lab products found in correlation
2 protocols using pe anti mouse foxp3 clone mf 14
1
Intrahepatic Lymphocyte Immunophenotyping
2
Multiparametric Flow Cytometry Panel
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Human TruStain FcX: clone 93, catalogue number 422302, Biolegend. TruStain FcX (anti-mouse CD16/32) Antibody: clone 93, catalogue number 101320, Biolegend. PE anti-human CD274 (B7-H1, PD-L1) Antibody: clone 29E.2A3, catalogue number 329706, Biolegend. FITC anti-human HLA-ABC Antibody: clone W6/32, catalogue number 311404, Biolegend. PE anti-mouse CD274 (B7-H1, PD-L1) Antibody: clone 10F.9G2, catalogue number 124308, Biolegend. FITC anti-mouse H2Kb Antibody: clone AF6-88.5, catalogue number 116506, Biolegend. FITC anti-mouse CD45: clone 30-F11, catalogue number 103108, Biolegend. Pacific blue anti-mouse CD3ε: clone 145-2c11, catalogue number 100334, Biolegend. Alexa Fluor647 anti-mouse CD4: clone GK1.5, catalogue number 100424, Biolegend. PE anti-mouse Foxp3: clone MF-14, catalogue number 126403, Biolegend. Zombie Aqua Fixable Viability Kit: catalogue number 423102, Biolegend. True-Nuclear Transcription Factor Buffer Set: catalogue number 424401, Biolegend. FVS780: catalogue number 565388, BD.
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