WBM was stained with cell surface markers as described. For intracellular Ki67/7AAD (cell cycle) staining, cells were fixed and permeabilized using Cytofix/Cytoperm kit according to manufacturer’s protocol (BD #554714) and stained with an anti-Ki67-BV605 (BioLegend #652413) for 1 hr at room temperature. 7AAD (BioLegend #420404) was added to samples and allowed to incubate for 20 min before analysis by flow cytometry. For AnnexinV apoptosis assay, WBM was washed twice with cold PBS and incubated at room temperature for 15 min in 1× binding buffer (10 mM HEPES, 140 mM NaCl and 2.5 mM CaCl2) containing AnnexinV– APC (eBiScience #88–8007-72) and 7AAD. Cells were analyzed by flow cytometry within 1 hr of staining.
Anti ki67 bv605
Manufactured by BioLegend
Sourced in United Kingdom
Anti-Ki67-BV605 is a fluorescently-labeled antibody that binds to the Ki67 protein, a reliable marker of cell proliferation. This product can be used in flow cytometry and other immunoassays to detect and quantify Ki67-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
7 aad, by BD (1 mentions)
7 aad, by BioLegend (1 mentions)
Cytofix cytoperm kit, by BD (1 mentions)
Efluor 506, by Thermo Fisher Scientific (1 mentions)
Efluor 450, by Thermo Fisher Scientific (1 mentions)
Anti pd 1 bv711, by BioLegend (1 mentions)
Anti cd44 buv395, by BD (1 mentions)
Mhcii, by Thermo Fisher Scientific (1 mentions)
Anti cxcr5 bv785, by BioLegend (1 mentions)
Anti cd4 buv805, by BD (1 mentions)
Cd4 apc alexa fluor 780, by Thermo Fisher Scientific (1 mentions)
Anti foxp3 pe cy7, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti ki67 bv605
1
Multiparameter Cell Cycle and Apoptosis Analysis
2
Multiparameter Analysis of Antigen-Specific CD4+ T Cells
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A single‐cell suspension was stained with PE‐labelled IAb/NP311–325 (NIH tetramer core) at 37°C, 5% CO2 for 2 hours in complete RMPI (RPMI with 10% foetal calf serum, 100 μg/ml penicillin‐streptomycin and 2 mM l ‐glutamine) containing Fc block (24G2). Surface antibodies were added, and the cells incubated for a further 20 minutes at 4°C. Antibodies used were as follows: anti‐CD4 BUV805 (BD Biosciences; clone: RM4‐5) or CD4 APC‐Alexa Fluor 780 (eBioscience; RM4‐5), anti‐CD44 BUV395 (BD Biosciences; clone: IM7), anti‐CXCR5 BV785 (BioLegend; clone:L138D7), anti‐PD‐1 BV711 (BioLegend: 29F.1A12) and ‘dump’ antibodies: B220 (RA3‐6B2), anti‐CD8 (53‐6.7) and MHC II (M5114) all on eFluor‐450 (eBioscience). Cells were stained with a fixable viability dye eFluor 506 (eBioscience). In some cases, cells were then fixed with FoxP3 Transcription Factor Fixative kit (Thermo Fisher, UK) and stained with anti‐FoxP3 PeCy7 (eBioscience; FJK‐16S), anti‐Bcl2 FITC (Biolegend; Blc/10C4) and anti‐Ki67 BV605 (Biolegend; 16A8). Phosphorylated H3 was detected in cells fixed with 2%PFA/0.5% saponin using Alexa 647‐labelled anti‐Histone H3 (pS28) (HTA28, Thermo Fisher). Cells were acquired on a BD LSR or Fortessa and analysed using FlowJo (version 10 Treestar).
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