Hcd45 fitc

After the cell count and viability were measured, isolated cells were washed with DPBS containing 0.2% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) and 1 mM ethylenediaminetetraacetic acid (EDTA; Gibco). About 1.5 × 10⁵ cells were mixed in each tube with the following antibodies: hCD31–fluorescein isothiocyanate (FITC) (clone: 5.6E; Beckman Coulter, Brea, CA, USA), hCD44–FITC (clone: G44-26; BD Biosciences, Franklin, NJ, USA), hCD45–FITC (clone: J33; Beckman Coulter), hCD73–FITC (clone: AD2; BD Biosciences), hCD81–FITC (clone: JS-81; BD Biosciences), hCD90–allophycocyanin (clone: 5E10; BD Biosciences), and CD105–phycoerythrin (clone: 266; BD Biosciences).
The cells were incubated for 90 min at 4 °C and then washed with DPBS containing 0.2% BSA and 1 mM EDTA. Fluoroprobe-labeled mouse IgG1 antibody (Beckman Coulter) and rat IgG2b antibody (BioLegend, San Diego, CA, USA) were used as negative controls. Stained cells were analyzed using a FACS Verse TM cell sorter (BD Biosciences).

After obtaining the cell count, isolated cells were washed with DPBS containing 0.2% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) and 1 mM ethylenediaminetetraacetic acid (EDTA; Gibco). About 1.5 × 10⁵ cells were mixed in each tube with the following antibodies: hCD31–fluorescein isothiocyanate (FITC) (clone: 5.6E, Beckman & Coulter, Brea, CA, USA), hCD44–FITC (clone: G44-26), hCD45–FITC (clone: J.33, Beckman & Coulter), hCD81–allophycocyanin (APC) (clone: JS-81, BD Bioscience, Franklin, NJ, USA), and hCD90–APC (clone: 5E10, BD Bioscience). The cells were incubated for 90 min at 4 °C and then washed with DPBS containing 0.2% BSA and 1 mM EDTA. Fluoroprobe-labeled mouse IgG1 antibody (clone: 679.1Mc7, Beckman & Coulter) and mouse IgG2b antibody (clone: MG2b-57, Beckman & Coulter) were used as negative controls. Stained cells were analyzed using a FACSVerse™ cell sorter (BD Bioscience).