All animal procedures complied with the animal care standards set forth by the US National Institutes of Health and have been approved by the Institutional Animal Care and Use Committee at the Institute of Neuroscience, Chinese Academy of Sciences. Mice were raised in a specific pathogen-free environment, group housed under a 12-12 h light-dark cycle, and with food and water provided at libitum from the cage lid. Health status was routinely checked and only naïve mice (no previous procedures or tests) were used. Wild-type ICR mice were used for IUE experiments; otherwise mice on C57/BL6 background were used. The day of vaginal plug was designated as embryonic day 0 (E0), while the day of birth was designated as postnatal day 0 (P0). Scnn1a-Tg3-Cre (full name: B6;C3-Tg (Scnn1a-cre)3Aibs/J; RRID:IMSR_JAX:009613) and Ai34D (full name B6;129S-Gt (ROSA)26Sor tm34.1(CAG-Syp/tdTomato)Hze /J; RRID:IMSR_JAX:012570) mice were obtained from the Jackson Laboratory, and crossed to generate Scnn1a-Tg3-Cre +/-::Ai34D +/+ mice. Both male and female P14 mice were used for all experiments. The numbers of mice used are as indicated in figure legends; 3 or more mice were used per experimental condition.
Ai34d
Manufactured by Jackson ImmunoResearch
Sourced in United States
The Ai34D is a laboratory instrument used for the detection and quantification of various biological molecules. It is a highly sensitive and accurate device designed to perform immunoassays, a technique that utilizes antibodies to identify and measure specific target analytes. The Ai34D is capable of analyzing a wide range of sample types, including cell cultures, tissue extracts, and biological fluids. This instrument provides researchers and scientists with a reliable and efficient tool for their analytical needs.
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3 protocols using ai34d
1
Tracing Cell-Type Specific Neural Circuits
2
Optogenetic Manipulation of Calretinin-Expressing Neurons
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Optogenetic studies were carried out on mice derived by crossing Calb2-IRES-cre (Jackson Laboratories, Bar Harbor, USA; #010774) with either Ai32 (Jackson Laboratories, Bar Harbor, USA; #024109) or Ai39 (Jackson Laboratories, Bar Harbor, USA, #014539) to generate offspring where ChR2/YFP or NpHR/YFP was expressed in CR+ cells (CRCre;Ai32 or CRCre;Ai39). Axon terminal labelling experiments crossed Calb2-IRES-cre and Ai34D (Jackson Laboratories, Bar Harbour, USA, # 012570) mice to generate offspring with CR+ axon terminals labelled with TdTomato. In control experiments, another transgenic mouse line with enhanced green fluorescent protein expressed under the control of the calretinin (Calb2) promoter (CReGFP) was used (Caputi et al., 2009 (link)). All experimental procedures were performed in accordance with the University of Newcastle’s animal care and ethics committee (protocols A-2013–312 and A-2016–603). Animals of both sexes were used for electrophysiology (age: 3–12 months) and behavior experiments (age: 8–12 weeks).
3
Transgenic Mouse Lines for Neuroscience
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