CLSM analyses of trophozoites were performed on a Leica TCS SP2 AOBS apparatus (Leica Microsystems, Germany) as described15. Antibodies used were: mouse polyclonal anti-gTrxR and anti-gEF1Bγ sera, 1:50; mouse anti-gADI and anti-gOCT4, 1:100; mouse anti-αTubulin mAb (clone B-5-1-2), 1:200; FITC-conjugated mouse anti-HA mAb (Miltenyi Biotec, Germany), 1:50; Alexa-Fluor 647- and 488-conjugated anti-rabbit and anti-mouse secondary Ab (Invitrogen), 1:500. Image deconvolution was performed with Huygens software (Scientific Volume Imaging BV, The Netherlands).
Alexa fluor 647 and 488 conjugated anti rabbit and anti mouse secondary ab
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
Alexa-Fluor 647- and 488-conjugated anti-rabbit and anti-mouse secondary antibodies are fluorescently-labeled secondary antibodies used in immunoassays and other applications. These antibodies bind to primary antibodies raised in rabbit or mouse, allowing for detection and visualization of target proteins or other biomolecules.
Automatically generated - may contain errors
2 protocols using alexa fluor 647 and 488 conjugated anti rabbit and anti mouse secondary ab
1
Confocal Microscopy Analysis of Trophozoites
2
Confocal Microscopy of Giardia duodenalis
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CLSM analyses of G. duodenalis trophozoites and encysting parasites were performed on a Leica TCS SP2 AOBS apparatus (Leica Microsystems, Wetzlar, Germany), as previously described [41 (link)]. The following antibodies were used: mouse polyclonal anti-GLV-capsid protein (GLV-CP) at 1:800 dilution; mouse monoclonal anti dsRNA (clone J2; Scicons, Hungary) at 1:100 dilution; rabbit polyclonal N14 (anti-g14-3-3) antiserum [40 (link)] at 1:100 dilution; mouse Cy5-conjugated anti-CWP mAb (Waterborne Inc., New Orleans, LA, USA), at dilutions of 1:20. Alexa-Fluor 647- and 488-conjugated anti-rabbit and anti-mouse secondary Ab (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA ), were used at 1:500 dilution. After staining, coverslips were extensively rinsed and then mounted, using Vectashield® mounting medium (Vector Laboratories Inc., Burlingame, CA, USA) containing 300 nM of 4′,6-diamidino-2-phenylindole (DAPI). Image deconvolution was performed by using Huygens 19.04 software (Scientific Volume Imaging BV, Hilversum, The Netherlands).
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