A small piece of tumor tissue from each mouse was collected, grounded, cracked, and centrifuged. The protein concentrations of each diluted sample were determined by using BCA assay kit. The total protein (40 μg) and MAKER were resolved by electrophoresis and transferred to PVDF membranes. The conditions for membrane transfer were as follows: VEGF, 200 mA and 70 min; caspase-3 and beta actin, 200 mA and 90 min. The membranes were blocked in TBST containing 5% skimmed milk for 2 hours at room temperature and then incubated with anti-caspase-3 antibody (1 : 800 dilution in TBST, Proteintech Group Inc., China) and anti-VEGF antibody (1 : 600 dilution in TBST, Bioworld, USA) overnight at 4°C. After five full washes in TBST, the membranes were incubated with anti-mouse IgG antibody labeled with horseradish peroxidase (1 : 50000 dilution in TBST, Boster, China) for 2 hours. The membrane was exposed to the X-ray film, which was rinsed, dried, and scanned, and then the grey value of the film was computed by using BandScan software.
Anti mouse igg antibody labeled with horseradish peroxidase
Manufactured by Boster Bio
Sourced in China
The Anti-mouse IgG antibody labeled with horseradish peroxidase is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various immunoassays. It consists of an anti-mouse IgG antibody covalently linked to the enzyme horseradish peroxidase, which serves as a reporter molecule for signal amplification.
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Lab products found in correlation
2 protocols using anti mouse igg antibody labeled with horseradish peroxidase
1
Western Blot Analysis of Apoptosis and Angiogenesis
2
Western Blot Analysis of Tumor Samples
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A small piece of tumor tissue from each mouse was collected, grounded, cracked, and centrifuged. The protein concentrations of each diluted sample were determined by using BCA assay kit. The total protein (40 μg) and MAKER were resolved by electrophoresis and transferred to PVDF membranes. The conditions for membrane transfer were: VEGF, 200 mA, and 70 min; Caspase-3 and beta-actin, 200 mA, and 90 min. The membranes were blocked in TBST containing 5% skimmed milk for 2 hours at room temperature and then incubated with anti-caspase-3 antibody (1:800 dilution in TBST, Protein tech Group Inc, China) and anti-VEGF antibody (1:600 dilution in TBST, Bioworld, USA) overnight at 4 °C. After five full washes in TBST, the membranes were incubated with anti-mouse IgG antibody labeled with horseradish peroxidase (1:50000 dilution in TBST, Boster, China) for 2 hours. The membrane was exposed to X-ray film, which was rinsed, dried, and scanned, after which the gray value of films was computed by using BandScan software.
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