Ti u inverted microscope

Manufactured by Oxford Instruments

The Ti-U inverted microscope is a versatile optical instrument designed for a range of applications in life science research and industrial applications. It features a stable inverted design with a large working distance, allowing for easy access to the sample. The microscope is equipped with a range of optical components, including objective lenses, illumination systems, and filters, enabling users to perform various imaging techniques such as brightfield, phase contrast, and fluorescence microscopy.

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Lab products found in correlation

Ixon ultra 897 ccd camera, by Oxford Instruments (1 mentions) Hpls245, by Thorlabs (1 mentions) Mf469 35, by Thorlabs (1 mentions) Mf525 39, by Thorlabs (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Glass coverslip, by Marienfeld (1 mentions) Et655lp, by Chroma Technology (1 mentions)

2 protocols using ti u inverted microscope

Analyzing Cyanobacterial Protein Localization

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Mid-log phase cultures of Synechocystis PCC 6803 and Cyanothece PCC 7425 cells harboring the pSB2TΔKm^R-gfp, pSB2T-ccmk1_tsbp₁-gfp and pSB2T-maf_S₆₈₀₃-gfp plasmids were placed in sandwiches consisting of two glass coverslips (22 mm diameter, Paul Marienfeld GmbH & Co. KG) one of which being coated with a Poly-L-lysine (Sigma-Aldrich) monolayer. These coverslip sandwiches were sealed and placed inside a home-made sample holder, which was mounted on a Nikon Ti-U inverted microscope coupled with an iXon ULTRA 897 CCD camera (Andor Technology), equipped with a 100x oil immersion (NA 1.45) microscope objective. Epifluorescence images were recorded using an excitation provided by a plasma light source (HPLS245 Thorlabs, Inc.) and an excitation filter (MF469-35 Thorlabs, Inc.), while for super-resolution laser scanning images a 488 nm laser (OBIS, Coherent) was used as an excitation source.
Chlorophyll and GFP fluorescence were recorded using ET655LP (Chroma Technology Corporation) and MF525/39 (Thorlabs, Inc.) emission filters, respectively.

Chenebault C., Diaz-Santos E., Kammerscheit X., Görgen S., Ilioaia C., Streckaite S., Gall A., Robert B., Marcon E., Buisson D.A., Benzerara K., Sassi J.F., Cassier-Chauvat C, & Chauvat F. (2020). A Genetic Toolbox for the New Model Cyanobacterium Cyanothece PCC 7425: A Case Study for the Photosynthetic Production of Limonene. Frontiers in Microbiology, 11, 586601.

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Visualizing Droplet Motion Dynamics

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To visualize the droplets, 0.5 mL of droplets were pipetted into a glass-bottom dish containing surfactant solution. Droplets were gently agitated to disperse them randomly within the dish. Videos of the droplet motion were taken using a Nikon Ti-U inverted microscope and an Andor Zyla 4.2P camera. Typically, several videos for each set of sample conditions were taken and analyzed to provide sufficient statistical data on droplet speeds and particle surface coverages. Fluorescence images were collected with excitation (AT480/30x) and emission (AT535/40m) filters.

Cheon S.I., Silva L.B.C., Khair A.S, & Zarzar L.D. (2021). Interfacially-adsorbed particles enhance the self-propulsion of oil droplets in aqueous surfactant. Soft matter, 17(28).

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