Hematoxylin–eosin (HE) and immunohistochemical staining were performed as previously described [19 (link)]. For immunofluorescence detection, the sections were routinely dewaxed and hydrated, and antigens were retrieved by microwave heating. Sections were treated with an autofluorescence quencher (Servicebio, Wuhan, China) and incubated with 5% (w/v) bovine serum albumin for 30 min at room temperature. Next, sections were incubated with primary antibody (1:100; Santa Cruz or 1:150, Proteintech) overnight at 4 °C. After being washed with PBS, the sections were incubated with FITC- or ALEX-conjugated goat anti-mouse IgG (1:200; Abcam, Waltham, MA, USA) at room temperature for 1–1.5 h in the dark. The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI). Finally, the antifluorescence quencher was used to seal the sections. Images were observed and obtained using a fluorescence microscope (LSM800, ZEISS, Oberkochen, Germany).
Fitc or alex conjugated goat anti mouse igg
Manufactured by Abcam
Sourced in United States
FITC- or ALEX-conjugated goat anti-mouse IgG is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is labeled with either fluorescein isothiocyanate (FITC) or Alexa Fluor 488 (ALEX) dye. It is used for the detection and visualization of mouse IgG in various immunoassays and imaging techniques.
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2 protocols using fitc or alex conjugated goat anti mouse igg
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Immunofluorescence Staining Protocol
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Immunofluorescence Staining Protocol
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The staining of Hematoxylin-Eosin (HE) and Immunohistochemistry was carried out as described [19] . For immuno uorescence, after routine dewaxing and hydration, antigen retrieval in sections was conducted by microwave heating. Sections were treated with an auto uorescence quencher (Servicebio, Wuhan, China), and closed with 5% (w/v) bovine serum albumin (BSA) for 30 minutes at room temperature. Next, sections were incubated with primary antibody (1:200; Santa cruz, USA or 1:150, Proteintech, China) overnight at 4 C. After being washed with PBS, sections were incubated with FITC or ALEX conjugated goat anti-mouse IgG (1:300; Abcam, USA) at room temperature for 1-1.5 h in the dark. The nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI). Finally, the anti-uorescence quencher was used to seal the sections. Images were observed and obtained using a uorescence microscope (LSM800, ZEISS, Oberkochen, Germany).
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