Alexa fluor

Manufactured by Bio-Rad

Alexa Fluor is a series of fluorescent dyes developed by Molecular Probes, a subsidiary of Thermo Fisher Scientific. These dyes are commonly used in fluorescence-based applications, such as immunohistochemistry, flow cytometry, and microscopy. Alexa Fluor dyes are known for their brightness, photostability, and wide range of excitation and emission wavelengths.

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Lab products found in correlation

Nitrocellulose membrane, by LI COR (1 mentions) Strep tactin hrp conjugate, by Bio-Rad (1 mentions) Horseradish peroxidase, by Bio-Rad (1 mentions) Mouse anti hcv ns3, by Abcam (1 mentions) Mouse anti flag tag, by Merck Group (1 mentions) Confocal laser scanning microscope, by Leica camera (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Bio-Rad (1 mentions)

3 protocols using alexa fluor

Exosome Protein Detection Assay

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Exosomes and A549 cells transiently transfected with viral plasmids were lysed with RIPA buffer. One μg of lysate was separated by 8%–15% SDS-PAGE and transferred to nitrocellulose membranes (LI-COR Biosciences) and blocked with 5% BSA in PBS at 4°C overnight. Strep fusion protein and CD63 were visualized by Strep-Tactin-HRP conjugate (Bio-Rad) and Alexa Fluor fluorescent-conjugated anti-CD63 antibody, respectively.

Teng Y., Xu F., Zhang X., Mu J., Sayed M., Hu X., Lei C., Sriwastva M., Kumar A., Sundaram K., Zhang L., Park J.W., Chen S.Y., Zhang S., Yan J., Merchant M.L., Zhang X., McClain C.J., Wolfe J.K., Adcock R.S., Chung D., Palmer K.E, & Zhang H.G. (2021). Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12. Molecular Therapy, 29(8), 2424-2440.

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Antibody Detection Protocol for Viral Proteins

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The following antibodies were used in this study: mouse anti-actin (EMD Milipore), mouse anti-HCV core (Affinity BioReagents), mouse anti-HCV NS3 (Abcam), mouse anti-Flag tag (Sigma), mouse anti-dsRNA (English Scientific Consulting), mouse anti-PDI (Stressgen), rabbit anti-HSD17B12 (Novus Biologicals), mouse anti-DENV NS3 (GeneTex; cross-reactive with ZIKV NS3) and rabbit DENV-NS4B (GeneTex; cross-reactive with ZIKV NS4B). Secondary antibodies coupled with horseradish peroxidase and Alexa Fluor were purchased from Bio-Rad and Invitrogen.

Mohamed B., Mazeaud C., Baril M., Poirier D., Sow A.A., Chatel-Chaix L., Titorenko V, & Lamarre D. (2020). Very-long-chain fatty acid metabolic capacity of 17-beta-hydroxysteroid dehydrogenase type 12 (HSD17B12) promotes replication of hepatitis C virus and related flaviviruses. Scientific Reports, 10, 4040.

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Immunofluorescence Staining Protocol

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The cells after treatment were washed by PBS and then xed by 4% paraformaldehyde for 30 min. The cells were permeabilized in 0.3% Triton X-100 for 30 min. After blocking with 3% BSA and incubating with primary antibodies, the cells were followed by incubation with a secondary antibody conjugated to Alexa Fluor (Bio-Rad) for 1 h and DAPI (Bio-Rad). The cell images were captured with a confocal laser scanning microscope (Leica).

Hu P., Wan P., Xu A., Yan B., Liu C., Xu Q., Wei Z., Xu J., Liu S., Yang G, & Pan Y. (2023). Neferine, a novel ROCK1-targeting inhibitor, blocks EMT process and induces apoptosis in non-small cell lung cancer. Journal of cancer research and clinical oncology, 149(2).

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