Healthy volunteers provided a nasal swab specimen. Each nostril was swabbed 10 times daily and collected into 100 ml of viral transport media (Corning; Corning, NY, USA). The nasal swab/viral transport media solution from all participants were pooled together to anonymise. The pooled negative background matrix was screened using the FilmArray RP2.1 to verify absence of 22 respiratory pathogens.
Viral transport media
Manufactured by Corning
Sourced in United States
Viral transport media is a sterile liquid medium designed to maintain the viability of viruses during sample collection, storage, and transport to a laboratory for testing. It provides a stable environment for viral particles, helping to preserve their integrity and prevent degradation.
Automatically generated - may contain errors
3 protocols using viral transport media
1
Nasal Swab Pooling for Pathogen Screening
2
SARS-CoV-2 RNA Extraction and RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MagMax Viral/Pathogen II (MVP II) Nucleic Acid isolation kit (Applied Biosystems, Foster City, CA) was used to extract nucleic acid (NA) from 200 μL of each specimen. A negative extraction control containing 200 μL viral transport media (Corning, Corning, NY) was included on every plate. Extractions were processed on the Kingfisher Flex Magnetic Particle Processor (Thermo Fisher Scientific Inc., Waltham, MA). The resulting elution volume was 50 μL and the same elution was used for testing with the EZ-SARS-CoV-2 RT-PCR and the TaqPath COVID-19 Combo Kit Multiplex Real-Time RT-PCR assays.
3
SARS-CoV-2 RNA Extraction and RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MagMax Viral/Pathogen II (MVP II) Nucleic Acid isolation kit (Applied Biosystems, Foster City, CA) was used to extract nucleic acid (NA) from 200 μL of each specimen. A negative extraction control containing 200 μL viral transport media (Corning, Corning, NY) was included on every plate. Extractions were processed on the Kingfisher Flex Magnetic Particle Processor (Thermo Fisher Scientific Inc., Waltham, MA). The resulting elution volume was 50 μL and the same elution was used for testing with the EZ-SARS-CoV-2 RT-PCR and the TaqPath COVID-19 Combo Kit Multiplex Real-Time RT-PCR assays.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!