Cd107 pe

Manufactured by BD

The CD107 PE is a lab equipment product that is used to detect the presence and quantify the levels of the CD107 protein in a sample. It is a fluorescently-labeled antibody that binds to the CD107 protein, allowing for its identification and measurement using flow cytometry or other analytical techniques.

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Lab products found in correlation

Live dead blue, by Thermo Fisher Scientific (1 mentions) Cd3 buv395, by BD (1 mentions) Cd56 apc, by BioLegend (1 mentions) Easysep human nk cell enrichment kit, by STEMCELL (1 mentions) Cd16 bv605, by BioLegend (1 mentions) Cd7 bv711, by BD (1 mentions) Cd4 apc, by BD (1 mentions) Cd4 fitc, by BD (1 mentions) Cd19 fitc, by BD (1 mentions) Cd4 bv421, by BioLegend (1 mentions) Cd16 apc h7, by BD (1 mentions) Cd8 bv421, by BD (1 mentions) Ir dye, by Thermo Fisher Scientific (1 mentions) Cd14 apc h7, by BD (1 mentions) Cd137 apc, by BD (1 mentions)

Spelling variants of this product

CD107 (3 citations) Anti-CD107-PE (3 citations)

2 protocols using cd107 pe

Characterization of NK Cell Activation

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Cryopreserved immune cells were thawed and enriched for NK cells using the EasySep™ Human NK Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA). Enriched NK cells were cultured with or without K562 cells at a 1:1 concentration for 4 hours with Golgi Stop added after 1 hour. Cells were then prepared for flow cytometry with the following markers: Live/Dead Blue (Invitrogen, Waltham, MA), CD56 APC, CD18 APC-Cy7, CD16 BV605 (Biolegend, San Diego, CA), CD3 BUV395, CD7 BV711, CD107 PE (BD, Franklin Lakes, NJ), and IFITM1 FITC (Biorbyt, St. Louis, MO).

Hilliard K.A., Throm A.A., Pingel J.T., Saucier N., Zaher H.S, & French A.R. (2022). Expansion of a novel population of NK cells with low ribosome expression in juvenile dermatomyositis. Frontiers in Immunology, 13, 1007022.

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Multiparametric Flow Cytometry Panel

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The following antibodies were used for flow cytometry: CD3-PerCP-Cy5.5 (clone SK7; eBioscience; used 1:20); CD4-FITC (clone RPA-T4; BD Biosciences; used 1:20), CD4-APC (clone RPA-T4; BD Biosciences; used 1:30), CD4-BV421 (clone SK3, Biolegend; used 1:100), CD8-BV421 (clone RPA-T8; BD Biosciences; used 1:50), CD14-APC-H7 (clone MoP9, BD Biosciences; used 1:100), CD16-APC-H7 (clone 3G8, BD Biosciences; used 1:100), CD19-FITC (clone 4G7, BD Biosciences; used 1:30), CD137-BV421 (clone 4B4-1; Biolegend; used 1:200), CD137-APC (clone 4B4-1; BD Biosciences; used 1:30), OX40-PE-Cy7 (clone Ber-ACT35, Biolegend), CD107-PE (clone H4A3, BD Biosciences; used 1:150) and PE-conjugated anti-mouse TCRβ constant domain (clone H57-597; BD Biosciences; used 1:150). The viability stain IR-Dye (Thermo Fisher, used 1:2,000) was used to identify live cells.

Cattaneo C.M., Battaglia T., Urbanus J., Moravec Z., Voogd R., de Groot R., Hartemink K.J., Haanen J.B., Voest E.E., Schumacher T.N, & Scheper W. (2023). Identification of patient-specific CD4+ and CD8+ T cell neoantigens through HLA-unbiased genetic screens. Nature Biotechnology, 41(6), 783-787.

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