Loss of neuron and pathological changes in hippocampus, cortex, and mesencephalon in cerebral I/R gerbils were presented by Nissl and hematoxylin–eosin (H&E) staining. After euthanasia, the brains were removed and placed in 4% paraformaldehyde for 24 h, then embedded in paraffin and sliced into 5‐μm‐thick sections. The Nissl bodies in neurons were stained purple‐red by the Nissl staining solution (Beyotime, C0017) for 10 min. For H&E staining, slices were dewaxed in water, stained for 5 min with hematoxylin, fractionated for a few seconds in 1% hydrochloric acid ethanol, and stained for 3 min with eosin. All slices were dehydrated and transparent. After being sealed with neutral gum and dried, they were photographed and analyzed using a microscope. Neuronal loss and pathological characteristics of slices were observed using an optical microscope (Leica DMI4000 B system).
Dmi4000 b system
Manufactured by Leica
Sourced in Germany
The DMI4000 B is a digital inverted microscope system designed for a variety of laboratory applications. It features a modular design, allowing for customization to meet specific user requirements. The system provides essential microscopy functionality without interpretation or extrapolation of its intended use.
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Lab products found in correlation
2 protocols using dmi4000 b system
1
Histopathological Analysis of Ischemic Injury
2
Transfection Efficiency Evaluation in Cell Lines
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HeLa cells, HEK293 cells, U251 cells and COS7 cells were separately seeded in 96-well plates (Corning Inc., Lowell, MA, USA) at a density of 5×10 3 cells/well on the day before transfection. When confluence reached 70-80%, the cells were transfected with PLL/DNA or PLL/DNA/peptide polyplexes containing 200 ng DNA and incubated for 4 h. The medium was then changed with fresh DMEM growth medium, and the cells were incubated for the reporter gene assays. Transfection mediated by Lipofectamine 2000 was performed following the manufacturer's instructions. Luciferase gene expression was measured at 24 h after transfection. Luciferase assays were performed with a Luciferase assay system, and relative light units (RLU) were measured with a 96 Microplate Luminometer (Glomax; Promega). Protein concentrations in the cell extracts were measured by a BCA assay. The final values were calculated as RLU/mg protein and reported as the mean ±SD obtained from triplicate transfections. HEK293 cells was used to evaluate the transfection of PLL/DNA/peptide polyplexes with pCMV-N-EGFP plasmid. Images were taken using Leica DMI 4000B system(Germany) under 10 × objective.
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