Mr plug play multi well hei standard

For the green synthesis of OMAgNs, 300 mL of C. intybus extracts were heated on a hot plate magnetic stirrer (MR Plug & Play Multi-Well Hei-Standard, Heidolph™) to 85 °C and stirred at ~1500 rpm for 15 min, then 5.1 g of silver nitrate (Sigma Aldrich™ Burlington, MA, USA) was added. After 10 min, 15 mL of concentrated hexane extract was added to the third flask (c) containing diluted 30 mL of methanol extract. The reactions were carried out at 65 °C for 3 h. The mixtures were then centrifuged at 10,000 rpm at 12 °C for 10 min (5810 R Eppendorf™, Hamburg, Germany) to collect the pellets. OMAgNs pellets were washed three times with ultrapure water (Milli-Q, Merck™, Burlington, MA, USA) and exposed to oven drying at 80 °C (Pol-Eko). As-synthesized methanolic (M-OMAgNs), acetone (A-OMAgNs), and methanol-hexane (MH-OMAgNs) were ground and subjected to further analysis.

Briefly, 180 g of C. intybus leaves and flowers were collected and washed with deionized H₂O. For extract preparation, plant material was finely ground in a mortar with liquid nitrogen, then divided into 3 parts, 60 g each. Each prepared plant powder was added to flasks containing one of the following: 300 mL of acetone (Avantor™, Gliwice, Poland), 300 mL of 99.8% methanol (Basic, Avantor™, Gliwice, Poland), and 300 mL of hexane (HPLC Grade, Sigma Aldrich™, Burlington, MA, USA). The mixtures were transferred to a hot plate magnetic stirrer (MR Plug & Play Multi-Well Hei-Standard, Heidolph™, Schwabach, Germany) and agitated at a speed of 1000 rpm for 20 min under ambient conditions. In the next step, the mixtures were placed in an ultrasonic bath (Sonorex Digiplus Badelin™, Berlin, Germany) for the next 20 min. The flasks were then placed undisturbed under ambient conditions for 30 min, following filtration using Whatman No. 1 filter paper. In the next step, the extracts were dried under vacuum at 40 °C using a rotary evaporator (Hei-Vap, Heidolph™, Germany) until the volume was reduced to 90 mL. Obtained extracts were divided into three flasks: (a) 45 mL of acetone extract with 255 mL ddH₂O; (b) 45 mL of methanol extract with 255 mL ddH₂O; (c) 30 mL of methanol extract with 255 mL ddH₂O. Finally, the mixtures were filtered using Whatman No. 1 filter paper.