Kh cb19

All procedures related to animal experimentation were reviewed and approved by the Kantonales Veterinäramt Basel-Stadt. Dissociated cultures of neocortical cells were prepared from E16.5 mouse embryos (embryonic stage 16.5). Neocortices were dissociated by the addition of papain (130 units, Worthington Biochemical, LK003176) for 30 min at 37°C. Cells (45,000 cells/cm2) were maintained in neurobasal medium (Gibco, 21103-049) containing 2% B27 supplement (Gibco, 17504-044), 1% Glutamax (Gibco, 35050-61), and 1% penicillin/streptomycin (Bioconcept, 4-01F00-H) for 14 days. The following reagents for pharmacological treatments were used (at the indicated concentrations and from the stated sources): DRB (50 µM, Sigma, D1916), triptolide (1 µM, Sigma, T3652), bicuculline (20 µM, Tocris, 0130), BDNF (50 ng/mL, Sigma, B7395), DHPG (10 µM, Tocris, 0805), DL-AP5 (50 µM, Tocris, 3693), KN-62 preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted October 14, 2021. ; https://doi.org/10.1101/2021.10.13.463312 doi: bioRxiv preprint -15 -(10 µM, Tocris, 1277), U0126 (10µM, Tocris, 1144), SRPIN340 (10 µM, Tocris, 5063) and KH-CB19 (10 µM, Tocris, 4262).