U mniba cube

FRET measurements were performed as previously described22 (link). Fluorescence images of Redoxfluor, HyPer, and roGFP were acquired using an IX70 fluorescence microscope (Olympus, Tokyo, Japan) equipped with a CoolSNAP HQ2 CCD camera (Photometrics, Tucson, AZ). HyPer and roGFP fluorescence was excited using the XF1076 filter (400AF30, Omega Optical, Brattleboro, VT) for excitation of the peak around 420 nm (400 nm for roGFP), and the U-MNIBA cube (BP 470–490, Olympus) for excitation of the peak around 500 nm (480 nm for roGFP); the corresponding YFP and GFP emissions were observed using the U-MNIBA cube (BP 515–550), respectively. Acquired images were analyzed using the MetaMorph imaging software (Universal Imaging Corp., Downingtown, PA).