The protein of cells or tumor tissues was extracted in lysis buffer (Beyotime Institute of Biotechnology), and then the concentrations were quantified according to the instructions of the Bicinchoninic Acid Protein Assay kit (Beyotime Institute of Biotechnology). Based on the concentration, 30 μg protein was isolated by 10% SDSpolyacrylamide separation gel before being transferred onto PVDF membranes. The membranes were then incubated with the primary antibodies [Tubulin, IDO, p-signal transducer and activator of transcription 3 (p-STAT3), signal transducer and activator of transcription 3 (STAT3), matrix metallopeptidase 2 (MMP2), cleaved caspase-3 or lc3b; Cell Signaling Technology, Inc.)], diluted according to the manufacturer's instructions. After 2 h, the membranes were washed with 1xTBST and incubated with the secondary antibodies for 1 h. Finally, the specificity of antigen-antibody complexes was detected using enhanced chemiluminescence reagent (Cell Signaling Technology, Inc.) and the images were visualized by Fusion FX Spectra imaging system.
Fusion fx spectra imaging system
Manufactured by Cell Signaling Technology
The Fusion FX Spectra imaging system is a high-performance instrument designed for sensitive, multimodal imaging of a wide range of samples. It incorporates a cooled, high-resolution CCD camera and supports a variety of imaging techniques, including chemiluminescence, fluorescence, and colorimetric detection.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using fusion fx spectra imaging system
1
Protein Extraction and Western Blot Analysis
2
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The protein of cells or tumor tissues was extracted in lysis buffer (Beyotime Institute of Biotechnology), and then the concentrations were quantified according to the instructions of the Bicinchoninic Acid Protein Assay kit (Beyotime Institute of Biotechnology). Based on the concentration, 30 μg protein was isolated by 10% SDS-polyacrylamide separation gel before being transferred onto PVDF membranes.
The membranes were then incubated with the primary antibodies [Tubulin, IDO, p-signal transducer and activator of transcription 3 (p-Stat3), signal transducer and activator of transcription 3 (Stat3), matrix metallopeptidase 2 (MMP2), cleaved caspase-3 or lc3b; Cell Signaling Technology, Inc.)], diluted according to the manufacturer's instructions. After 2 h, the membranes were washed with 1xTBST and incubated with the secondary antibodies for 1 h. Finally, the specificity of antigen-antibody complexes was detected using enhanced chemiluminescence reagent (Cell Signaling Technology, Inc.) and the images were visualized by Fusion FX Spectra imaging system.
The membranes were then incubated with the primary antibodies [Tubulin, IDO, p-signal transducer and activator of transcription 3 (p-Stat3), signal transducer and activator of transcription 3 (Stat3), matrix metallopeptidase 2 (MMP2), cleaved caspase-3 or lc3b; Cell Signaling Technology, Inc.)], diluted according to the manufacturer's instructions. After 2 h, the membranes were washed with 1xTBST and incubated with the secondary antibodies for 1 h. Finally, the specificity of antigen-antibody complexes was detected using enhanced chemiluminescence reagent (Cell Signaling Technology, Inc.) and the images were visualized by Fusion FX Spectra imaging system.
3
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protein of cells or tumor tissues was extracted in lysis buffer (Beyotime Institute of Biotechnology), and then the concentrations were quantified according to the instructions of the Bicinchoninic Acid Protein Assay kit (Beyotime Institute of Biotechnology). Based on the concentration, 30 μg protein was isolated by 10% SDS-polyacrylamide separation gel before being transferred onto PVDF membranes.
The membranes were then incubated with the primary antibodies [Tubulin, IDO, p-signal transducer and activator of transcription 3 (p-Stat3), signal transducer and activator of transcription 3 (Stat3), matrix metallopeptidase 2 (MMP2), cleaved caspase-3 or lc3b; Cell Signaling Technology, Inc.)], diluted according to the manufacturer's instructions. After 2 h, the membranes were washed with 1xTBST and incubated with the secondary antibodies for 1 h. Finally, the specificity of antigen-antibody complexes was detected using enhanced chemiluminescence reagent (Cell Signaling Technology, Inc.) and the images were visualized by Fusion FX Spectra imaging system.
The membranes were then incubated with the primary antibodies [Tubulin, IDO, p-signal transducer and activator of transcription 3 (p-Stat3), signal transducer and activator of transcription 3 (Stat3), matrix metallopeptidase 2 (MMP2), cleaved caspase-3 or lc3b; Cell Signaling Technology, Inc.)], diluted according to the manufacturer's instructions. After 2 h, the membranes were washed with 1xTBST and incubated with the secondary antibodies for 1 h. Finally, the specificity of antigen-antibody complexes was detected using enhanced chemiluminescence reagent (Cell Signaling Technology, Inc.) and the images were visualized by Fusion FX Spectra imaging system.
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