The cells were seeded in 22 mm confocal dishes at an appropriate density. After LPS priming and 3% DSS, ERB-041 (1 mM) and TNFa (5 ng/mL) (Z01001-10, Genscript) or IL-1b (10 ng/mL) (Z02922-10, Genscript) stimulation, the cells were incubated for 30 min at 37 C with 250 nM MitoTracker Red CMXRos Mitochondrial Probe (M7512, Thermo Fisher Scientific). The fluorescence signals were measured by Zeiss microscope (excitation at 579 nm; emission at 599 nm).
Mitotracker red cmxros mitochondrial probe
Manufactured by Thermo Fisher Scientific
MitraTracker Red CMXRos Mitochondrial Probe is a fluorescent dye that selectively accumulates in active mitochondria. It is used to visualize and study mitochondrial structure and function in living cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mitotracker red cmxros mitochondrial probe
1
Mitochondrial Dynamics in Inflammatory Response
2
Mitochondrial Dynamics in Inflammatory Response
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The cells were seeded in 22 mm confocal dishes at an appropriate density. After LPS priming and 3% DSS, ERB-041 (1 μM) and TNF-α (5 ng/mL) (Z01001-10, Genscript)
or IL-1β (10 ng/mL) (Z02922-10, Genscript) stimulation, the cells were incubated for 30 min at 37 °C with 250 nM MitoTracker® Red CMXRos Mitochondrial Probe (M7512, Thermo Fisher Scientific). The fluorescence signals were measured by Zeiss microscope (excitation at 579 nm; emission at 599 nm).
or IL-1β (10 ng/mL) (Z02922-10, Genscript) stimulation, the cells were incubated for 30 min at 37 °C with 250 nM MitoTracker® Red CMXRos Mitochondrial Probe (M7512, Thermo Fisher Scientific). The fluorescence signals were measured by Zeiss microscope (excitation at 579 nm; emission at 599 nm).
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