EESP stock solution was prepared as previously described [15 (link)]. Human carcinoma HCT-8 cells and HCT-8/5-FU cells (KeyGEN Biotech, Nanjing, China) were cultured at 37°C in a humidified incubator with 5% CO2 in RPMI medium 1640 (KeyGEN Biotech, Nanjing, China) supplemented with 10% FBS (Hyclone, Carlsbad, USA), 100 U/mL of penicillin, and 100 μg/mL of streptomycin (Life Technologies, Shanghai, China). HCT-8/5-FU cells were cultured in the abovementioned medium with the addition of 15 μg/mL of 5-FU (Shanghai Amino Acids Company, Shanghai, China).
Hct 8 5 fu cells
Manufactured by Keygen Biotech
Sourced in China
HCT-8/5-FU cells are a type of human colorectal adenocarcinoma cell line. They are commonly used in cell-based assays and research related to colorectal cancer.
Automatically generated - may contain errors
3 protocols using hct 8 5 fu cells
1
Cultivation of Human Carcinoma Cell Lines
2
CRC Cell Line Maintenance Protocol
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Human CRC HCT-8 cells and 5-FU resistant HCT-8/5-FU cells were obtained from Nanjing KeyGen Biotech. Co., Ltd. Cells were grown in RPMI-1640 medium, supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin. In addition, HCT-8/5-FU cell cultures were supplemented with 15 µg/ml 5-FU. Cells were cultured at 37°C and 5% CO2 in a humidified incubator. The cells were subcultured at a ratio of 1:4-1:6 at between 80 and 90% confluency.
3
Culturing Human Colorectal Carcinoma Cells
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Human colorectal carcinoma HCT-8 cell and 5-FU resistant HCT-8/5-FU cells were obtained from Nanjing KeyGen Biotech. Co. Ltd. (Nanjing, Jiangsu, China). Cells were grown in RPMI 1640 medium containing 10% (v/v) FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin in a 37°C humidified incubator with 5% CO2. The cells were subcultured at 80–90% confluency. HCT-8/5-FU cells were cultured in RPMI-1640 containing 15 μg/mL of 5-FU.
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