Bench top scanning electron microscope prox

Ultraviolet-visible spectroscopic analysis was conducted in a UV-1900-UV-Visible spectrophotometer from Shimadzu (Japan). Chiral gas chromatography (GC) was performed on a GC-14B equipped with flame ionization detector and a CP-Chirasil-Dex-CB column (Varian 0.32 mm × 0.25 μm × 50 m) using He carrier gas (5 mL min⁻¹, head pressure: 274 kPa, injector: 180 °C, detector: 180 °C) (Shimadzu, Japan). The morphology and energy dispersive X-ray spectroscopy (EDX) analysis were studied by Bench-top Scanning Electron Microscope proX supplied by Phenom-World (Netherlands). Thermo Gravimetric Analysis (TGA) was performed on TG-DTA, DTG-60 (Shimadzu, Japan). The optical rotation value was measured by JASCO P-2200 Polarimeter with a 10 cm path-length cell. The ¹H-NMR analysis was performed on a Bruker Biospin AVANCE III 400 spectrometer at 400 MHz in CDCl₃.

Recombinant E. coli strains, Rosetta TM(DE3)pLysS-pET21b(+)-TaGDH (Ta0897, KEGG) and BL21(DE3)pLysS-pET21b(+)-TaIDH (Ta0117, KEGG) constructed in our previous study [22 (link)], were used. The reagents were purchased from commercial sources, including Nacalai Tesque (Japan), Wako Pure Chemical Industries (Japan), Tokyo Chemical Industry Co. (Japan), Sigma-Aldrich (USA), and Bio-Rad (USA), and were used without further purification. Scanning electron microscope (SEM) analysis was conducted using a benchtop scanning electron microscope proX from Phenom-World (Netherlands), as reported previously [15 (link)].