Milliplex map human cytokine chemokine growth factor panel a 48 plex

Mock and infected organoids were centrifuged, and supernatant was collected. All supernatants were mixed with 10% Triton-X100 to a final concentration of 1% Triton-X100 for inactivation of virus, decontaminated from the BSL3 facility and frozen at −80°C. Supernatants were analyzed neat and in duplicate using MILLIPLEX^® MAP Human Cytokine/Chemokine/Growth Factor Panel A 48 Plex (Millipore Sigma, HCYTA-60K-PX48) by the Human Immune Monitoring Core at Stanford University.

Cytokine concentrations in plasma were measured using the MILLIPLEX MAP Human Cytokine/Chemokine/Growth Factor Panel A (48 Plex) (Millipore Sigma, St. Louis MO, cat no. HCYTA-60K-PX48) by the Flow Cytometry Facility of UVA. The cytokines detected were sCD40L, epidermal growth factor (EGF), eotaxin, fibroblast growth factor 2, Flt-3 ligand, fractalkine, granulocyte colony-stimulating factor, GM-CSF, GROα, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1 receptor antagonist, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10, MCP-1, MCP-3, M-CSF, macrophage-derived chemokine (MDC/CCL22), MIG, MIP-1α, MIP-1β, platelet-derived growth factor (PDGF)-AA, PDGF-AB/BB, transforming growth factor α, TNF-α, TNF-β, and vascular endothelial growth factor A. (RANTES was excluded).