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Sodium bicarbonate

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Sodium bicarbonate is a white crystalline powder that is commonly used as a laboratory reagent. It has the chemical formula NaHCO3 and is a salt of carbonic acid. Sodium bicarbonate is a versatile compound that can be used for various purposes in a laboratory setting.

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Lab products found in correlation

Sodium nitrite solution, by Merck Group (1 mentions) Diphenyleneiodonium, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dulbecco s modified eagle medium dmem low glucose, by Merck Group (1 mentions) Penicillin streptomycin mixture, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Griess reagent, by Merck Group (1 mentions) Caco 2 cell, by American Type Culture Collection (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions) Sodium bicarbonate, by Thermo Fisher Scientific (1 mentions) Methanol d4, by Merck Group (1 mentions) Dimethyl sulfoxide d6, by Merck Group (1 mentions) β glycerophosphate β gp, by Merck Group (1 mentions) Alizarin red s, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Avantor (1 mentions) Triton x 100, by Avantor (1 mentions)

3 protocols using sodium bicarbonate

1

Evaluating Inflammatory Response in Cells

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Diphenyleneiodonium (DPI), 2′,7′-dichlorofluorescin diacetate (DCFA), lipopolysaccharide (LPS), sodium nitrite solution, Griess reagent, Dulbecco’s Modified Eagle Medium (DMEM)/low Glucose, phenol red-free DMEM/low Glucose, penicillin/streptomycin mixture, DMSO, and fetal bovine serum (FBS) were purchased from Sigma (St. Louis, MO, USA). Glucose was purchased from Acros Organics (Fair Lawn, NJ, USA), and sodium bicarbonate was purchased from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). Caco-2 cells and RAW 264.7 macrophages (cell line TIB-71™) were acquired from the American Type Culture Collection (ATCC) (Manassas, VA, USA).
Sreedharan S., Nair V, & Cisneros-Zevallos L. (2023). Protective Role of Phenolic Compounds from Whole Cardamom (Elettaria cardamomum (L.) Maton) against LPS-Induced Inflammation in Colon and Macrophage Cells. Nutrients, 15(13), 2965.
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2

Photostable Cy3 Fluorophore Labeling Protocol

Cited 1 time
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A 10 mM pH 7 phosphate buffered saline (PBS, NaH2PO4•H2O: Na2HPO4 = 1:3.4 without additional salt) and a 10 mM pH 5 sodium acetate buffer were prepared and filtered through a disposable Millex-GP syringe filter unit (0.22 μm) before use. Glass cover slips (20 mm × 20 mm square, Karl Hecht, Sondheim, Germany); potassium dichromate (ICN Biomedicals Inc. Aurora, Ohio); deionized (DI) water (18.2 MΩ, 0.22 μm pore size filter, APS Water Services Corp., Van Nuys, CA) were used for all experiments. A 50 mM 1-(3-aminopropyl) silatrane (APS) stock solution was prepared by dissolving the APS powder in DI water. The intra-molecularly photostabilized Cy3 fluorophore was chemically synthesized20 (link). Maleimide-poly (ethylene glycol)-succinimidyl valerate (Mal-PEG-SVA; 3.4 kDa) and methoxy-poly (ethylene glycol)-succinimidyl valerate (mPEG-SVA, 2 KDa) were purchased from Laysan Bio Inc, Arab, AL. Additional reagents included: sodium bicarbonate (Mallinckrodt Baker Inc, Paris, Kentucky); sodium hydroxide (GR grade, EM science, Darmstadt, Germany); sodium phosphate monobasic (ACS grade, Fisher Scientific, Fair Lawn, NJ); sodium phosphate dibasic anhydrous (ACS grade, Fisher Scientific, Fair Lawn, NJ); Amicon 10 kDa and 30 kDa centrifugal filter units (EMD Millipore Co. Billerica, MA); and a 0.1-mm-thick teflon spacer (American Durafilm, Holliston, MA).
Lv Z., Krasnoslobodtsev A.V., Zhang Y., Ysselstein D., Rochet J.C., Blanchard S.C, & Lyubchenko Y.L. (2016). Effect of acidic pH on the stability of α–Synuclein dimers. Biopolymers, 105(10), 715-724.
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3

Cell Viability and Mineralization Assays

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Alizarin red S, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide-d6 (DMSO-d6), β-glycerophosphate (β-GP), and methanol-d4 (Sigma-Aldrich, St. Louis, MO, USA). Cetylpyridinium chloride, p-nitrophenyl phosphate, sodium bicarbonate, and sodium phosphate (Mallinckrodt, St. Louis, MO, USA). Dulbecco′s phosphate-buffered saline (PBS) (Gibco, Burlington, ON, Canada). Dimethyl sulfoxide (DMSO), and Triton-X 100 (J-T Baker, Phillipsburg, NJ, USA). ACS-grade ethanol and methanol (MeOH) Echo chemical, Miaoli, Taiwan). p-Nitrophenyl phosphate, sodium bicarbonate, and paraformaldehyde (Alfa Aesar, Ward Hill, MA, USA).
Imtiyaz Z., Wang Y.F., Lin Y.T., Liu H.K, & Lee M.H. (2019). Isolated Compounds from Turpinia formosana Nakai Induce Ossification. International Journal of Molecular Sciences, 20(13), 3119.
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