293t human embryonic kidney cell line atcc crl 3216

DOTAP were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) protamine sulfate was purchased Sigma-Aldrich (St Louis, MO). All the other chemicals were purchased from Sigma-Aldrich unless otherwise mentioned. mMESSAGE mMACHINE™ T7 Transcription Kit and an MEGAclear™ Transcription Clean-Up Kit, OptiMem®, Lipofectamine® 3000, Dulbecco's modified Eagle's medium (DMEM) and serums were purchased from Thermo Fisher Scientific. CT26 Mus musculus colon carcinoma cell line (ATCC® CRL-2638™) and 293t human embryonic kidney cell line (ATCC® CRL-3216™) were purchased from the American Type Culture Collection (ATCC). The plasmid pVAX1-VSVMP expressing vesicular stomatitis virus matrix protein has been described previously.^{10 (link)} All plasmids were propagated in E. coli and purified by an EndoFree Plasmid Giga kit (Qiagen, Chatsworth, CA). BALB/c mice were obtained from Beijing HFK Bio-technology Co. Ltd. (Beijing, China) and maintained under specific pathogen-free conditions. All animal procedures were approved and controlled by the Institutional Animal Care and Treatment Committee of Sichuan University and carried out according to the Animal Care and Use Guidelines of Sichuan University.