Total RNA was extracted using TRIzol reagent (Invitrogen, CA) according to the manufacturer’s instructions. Reverse transcription reactions were performed with 1 μg of total RNA using FastQuant RT kit (TIANGEN Biotech, Beijing, China). The sequence of the miR-30d-5p stem loop is 5ʹ-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTCCA-3ʹ. GLDC expression was quantified using a SYBR qPCR Kit (TIANGEN Biotech) according to the manufacturer’s instructions. All samples were run in triplicate. The endogenous RNA reference gene used was 18s ribosomal RNA. The relative expression levels were evaluated using the 2−ΔΔCt method20 (link). Primers are listed in Supplementary Table S1 .
Sybr qpcr kit
Manufactured by Tiangen Biotech
Sourced in China
The SYBR qPCR Kit is a real-time PCR reagent kit that utilizes SYBR Green dye to detect and quantify DNA sequences. It enables the amplification and detection of target DNA in a single reaction. The kit provides the necessary components, including a master mix, primers, and controls, to perform quantitative PCR analysis.
Automatically generated - may contain errors
2 protocols using sybr qpcr kit
1
Quantifying miR-30d-5p and GLDC Expression
2
RNA Extraction and qPCR Analysis of DDX27 and MVP
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell pellets were harvested. Total RNA was extracted using Trizol (Invitrogen) according to manufacturer's protocol. Complementary single-strand DNA (cDNA) was transcripted using FastQuant RT kit (With gDNA, TIANGEN, Beijing, China). The expression levels of DDX27 and MVP were determined by qPCR using SYBR qPCR Kit (TIANGEN, Beijing, China). The primer sequences are listed in Table S1 .
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