Monoclonal antibodies to β actin

Polyclonal antibody to CD44 (ab41478) was obtained from Abcam (Seoul, Korea). Polyclonal antibodies to AKT (9272S), p-AKT (S473) (9271S), p-AKT (T308) (9275S), SNAIL (3879), P38 (9121), and p-p38 (9211S) were obtained from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibodies to vimentin (sc-5565), SLUG (SC-10436), TWIST (sc-15393), c-SRC (sc-19), Lyn (sc-15), Fyn (sc-16), LCK (sc-13), and SOX2 (sc17319) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibody to ZEB1 (HPA027524) was purchased from Sigma (St. Louis, MO, USA). Polyclonal antibodies to N-cadherin (610920) and E-cadherin (612130) were purchased from BD Transduction Laboratory (Seoul, Korea). Monoclonal antibodies to β-actin and DAPI were purchased from Sigma. Etoposide (341205), cisplatin (232120), and LY294002 a chemical inhibitor specific to PI3K (440202), were purchased from Calbiochem (San Diego, CA, USA). Paclitaxel (T1912-5MG) was purchased from Sigma.

Polyclonal antibodies to phospho-AKT (Ser-473), phospho-AKT (Thr-308), phospho-ERK1/2 (Thr-202/Tyr-204), ERK and N-cadherin were obtained from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibodies to AKT, Fibronectin (FN1), TWIST, ZEB1, SNAIL and SLUG were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The polyclonal antibody Vimentin (VIM) was obtained from Thermo Scientific (Seoul, Korea). 4,6-Diamidino-2-phenylindole and monoclonal antibodies to β-actin were obtained from Sigma (St Louis, MO, USA). Anti-mouse Alexa Fluor 488, and anti-rabbit Alexa Fluor 488 were purchased from Invitrogen. E-cadherin was obtained from BD Biosciences (San Jose, CA, USA). KRAS was purchased from Abcam (Seoul, Korea).