Sodium pentobarbital

Manufactured by Thermo Fisher Scientific

Sodium pentobarbital is a sedative-hypnotic pharmaceutical compound. It is a barbiturate drug used as a general anesthetic, anticonvulsant, and for the treatment of insomnia. The core function of sodium pentobarbital is to induce sedation and reduce neural activity in the central nervous system.

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Lab products found in correlation

Normal donkey serum (nds), by Thermo Fisher Scientific (1 mentions) N2 supplement, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Normal goat serum (ngs), by Merck Group (1 mentions) Normal donkey serum (nds), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)

2 protocols using sodium pentobarbital

Reagent Sources for Neurophysiology Research

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Reagents were obtained from the following sources: Abbott (Chicago, IL): sodium pentobarbital (0074-378-05); Affymetrix (Santa Clara, CA): HEPES, pH 7.3 (16924); Calbiochem (San Diego, CA): tetrodotoxin (584411); Jackson ImmunoResearch (West Grove, PA): normal donkey serum (017000121); Life Technologies: L-glutamine (25030-081), N-2 Supplement (17502-048), penicillin/streptomycin (15140-122), phosphate-buffered saline (Ca²⁺- and Mg²⁺-free, pH 7.4; 70011-044); Tris (pH 8.0; AM9856); Sigma-Aldrich (St. Louis, MO): bovine serum albumin (A8806), horse serum (H1138), sodium phosphate dibasic (RES20908), sodium phosphate monobasic (71505); Thermo Fisher Scientific: Triton X-100 (BP151-100); Tocris Bioscience (Minneapolis, MN): CNQX [6-cyano-7-nitroquinoxaline-2,3-dione] (0190), L-AP4 [L-(+)-2-amino-4-phosphono butyric acid] (0103), (RS)-CPP [(RS)-3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid] (0173).

Stradleigh T.W., Greenberg K.P., Partida G.J., Pham A, & Ishida A.T. (2014). Moniliform Deformation of Retinal Ganglion Cells by Formaldehyde-Based Fixatives. The Journal of comparative neurology, 523(4), 545-564.

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Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were obtained from the following sources: Abbott (Chicago, IL): sodium pentobarbital (0074-378-05); Affymetrix (Santa Clara, CA): low melt agarose (32829), HEPES, (pH 7.3, 16924); Calbiochem (San Diego, CA): FluorSave (345789); Corning (Corning, NY): Tris-HCl, pH 7.5 (46-030CM); Fisher Scientific (Pittsburgh, PA): PBS (Ca²⁺- and Mg²⁺-free, pH 7.4; MT21040CV); Invitrogen (Grand Island, NY): DAPI (D21490); Jackson ImmunoResearch: normal donkey serum (017000121), normal goat serum (005000121); Sigma-Aldrich (St. Louis, MO): BSA (A8806), sodium azide (S2002); Thermo Fisher Scientific: TO-PRO-3 (T3605), Triton X-100 (BP151-100); United States Biological (Salem, MA): Ames (A1372-25); Western Medical Supply (Arcadia, CA): ketamine (4165), xylazine (5533).

Fasoli A., Dang J., Johnson J.S., Gouw A.H., Iseppe A.F, & Ishida A.T. (2017). Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina. The Journal of comparative neurology, 525(7), 1707-1730.

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