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Enhanced chemiluminescence

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Enhanced chemiluminescence is a laboratory equipment used to detect and quantify specific proteins or other biomolecules in a sample. It is based on the principle of chemiluminescence, where a chemical reaction emits light that can be measured and analyzed. The equipment is designed to provide a sensitive and reliable method for protein detection and analysis.

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Lab products found in correlation

X ray film, by Kodak (2 mentions) Mouse monoclonal anti gapdh, by Merck Group (1 mentions) Rabbit polyclonal anti bdnf, by Santa Cruz Biotechnology (1 mentions) Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions) Bcl 2, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse secondary antibody, by Santa Cruz Biotechnology (1 mentions) Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions) Bca assay, by Bio-Rad (1 mentions) Mini protean tgx precast gel, by Bio-Rad (1 mentions)

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Enhanced chemiluminescence kit Enhanced chemiluminescence system Enhanced chemiluminescence reagents

3 protocols using enhanced chemiluminescence

1

Hippocampal Protein Expression Analysis

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Hippocampi were dissected from animal after the end of LBP and SCO administration. Protein was extracted using a mammalian Protein Extraction Reagent (Pierce, Rockford, IL, USA) according to the manufacturer’s instructions and separated on 10% polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS). Membranes after transferring were subsequently blocked with 5% non-fat milk in TBS and incubated with mouse monoclonal anti-IGF-1 (1∶1,000, Sigma, St Louis, MO), rabbit polyclonal anti-BDNF (1∶200), -Bax (1∶500), -Bcl-2 (1∶500, Santa Cruz, CA, USA) and mouse monoclonal anti-GAPDH (1∶10,000, Sigma, St Louis, MO, USA) overnight at 4°C. Membranes were developed by incubation with horseradish peroxidase-conjugated goat anti-mouse or rabbit IgG (1∶3,000, Pierce, Rockford, IL, USA) for 2 h at room temperature. After washing, the complexes were visualized by enhanced chemiluminescence (Santa Cruz, CA, USA) and exposed to X-ray film (Kodak, Rochester, NY, USA). The intensity of each band was quantified using the Shine-tech Image System (Shanghai, CHN).
Chen W., Cheng X., Chen J., Yi X., Nie D., Sun X., Qin J., Tian M., Jin G, & Zhang X. (2014). Lycium barbarum Polysaccharides Prevent Memory and Neurogenesis Impairments in Scopolamine-Treated Rats. PLoS ONE, 9(2), e88076.
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2

BDNF Protein Expression Analysis

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Total protein was extracted from either infected ADSCs or uninfected ADSCs. Lysate containing 20 μg of protein was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotted. The membrane was sequentially incubated with monoclonal mouse anti-rat BDNF antibody (1:500; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-GAPDH antibody (1:1000; Santa Cruz Biotechnology) at 37°C for 24 hours. Following goat anti-mouse secondary antibody (Santa Cruz Biotechnology) incubations at the ambient temperature for 1 hour, signals were visualized by enhanced chemiluminescence (Eastman Kodak, Rochester, NY, USA).
Yang M., Sun J.Y., Ying C.C., Wang Y, & Guo Y.L. (2019). Adipose-derived stem cells modified by BDNF gene rescue erectile dysfunction after cavernous nerve injury. Neural Regeneration Research, 15(1), 120-127.
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3

Western Blot Analysis of Oyster Proteins

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Cells were lysed with ice cold 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% v/v Triton X, protease inhibitor (1:100), 0.1 mM PMSF. Concentration of proteins in lysates was determined by standard BCA assay (Bio-Rad). Equivalent levels of protein from different tissues of oyster were subjected to electrophoresis on 4–15% mini-protean TGX precast gels (Bio-Rad, #456-1084) and transferred onto PVDF membrane (PRC #88518). The membranes were blocked in 5% nonfat milk and probed with primary antibodies at 1:3000 dilutions. Next, membranes were washed in PBS-T followed by incubation with HRP-linked secondary rabbit polyclonal antibody (1:3000) and the bands were visualized with enhanced chemiluminescence (ECL) reagents following exposure of membranes to x-ray film (Kodak, Rochester, NY).
Feng C., Ghosh A., Amin M.N., Bachvaroff T.R., Tasumi S., Pasek M., Banerjee A., Shridhar S., Wang L.X., Bianchet M.A, & Vasta G.R. (2015). The Galectin CvGal2 from the Eastern Oyster (Crassostrea virginica) Displays Unique Specificity for ABH Blood Group Oligosaccharides and Differentially Recognizes Sympatric Perkinsus Species. Biochemistry, 54(30), 4711-4730.
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