Cell shape was analysed on fixed samples (4% PFA, Sigma-Aldrich, Arklow, Ireland). After fixation, samples were thoroughly washed in PBS and permeabilised using 0.5% Triton-X. Fluorescent stains were diluted in 1.5% BSA solution (for rhodamine phalloidin (ex/em = 540/565 nm) at 1:40, and for DAPI (ex/em = 364/454 nm), a 1:1000 dilution was used). Stains were incubated for 1 h (rhodamine phalloidin) and 10 min (DAPI) at room temperature, avoiding artificial light, imaged with a Leica SP8 scanning confocal at 358/524 and 540/565 nm channels, and assessed using Leica Application Suit X (LAS X) Software.
Application suit x las x software
Manufactured by Leica
Leica Application Suite X (LAS X) Software is a comprehensive microscope imaging and analysis software platform developed by Leica Microsystems. It provides a user-friendly interface for controlling various Leica microscope models and accessories, as well as tools for image acquisition, processing, and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using application suit x las x software
1
Cell Shape Analysis by Confocal Microscopy
2
Microencapsulated BMSC Histology in Bovine Discs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Healthy, unprimed and primed microencapsulated-BMSC-treated bovine discs were stained for cell morphology after 28 d of whole organ culture. Briefly, slides were permeabilised using 0.5 % Triton X-100 and stained with rhodamine phalloidin at 200 U/ mL (diluted 1 : 40; Sigma-Aldrich) to stain the actin cytoskeleton. 4′,6′-diamidino-2-phenylindole (DAPI, diluted 1 : 500; Sigma-Aldrich) was used to stain the nucleus and rinsed with PBS. Subsequently, stained slides were imaged with a Leica SP8 scanning confocal at 358/524 and 540/565 nm wave-length (40× magnification) and assessed using the Leica Application Suit X (LAS X) software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!