Trimyristin (Dynasan 114), was donated by Cremer® (Hamburg, Germany). Egg lecithin E80 was purchased by Lipoid® (Ludwigshafen, Germany). The material used as a conjugated polymer (CN-PPV), Nile red, wheat germ agglutinin conjugated with fluorescein (WGA-FITC), surfactant (PEG-660 stearate), castor oil (CO), Dulbecco’s Modified Eagle’s Medium (DMEM), quercetin (QU), porcine gastric mucin (type II), rat red cell suspension, N-acetylglucosamine, PBS-BSA solution, antibody anti-WGA, anti-rabbit IgG produced in goat, dialysis bag MWCO 10000, and Ortho-Phenylene-Diamine were purchased by Sigma-Aldrich (Saint Louis, MO, EUA). The modified N-Acetyl-b-D-glucosaminyl-PEG900-stearate conjugate (C18PEG900GlcNAc) was synthesized by Alexandre G. Dal Bó [10 (link),11 (link),23 (link)]. WGA lectins were extracted by Luciano Pinto. The other reagents used were of analytical grade.
Egg lecithin e80
Manufactured by Lipoid
Sourced in Germany
Lipoid E80 is a refined egg lecithin product. It is a naturally derived material composed of phospholipids, primarily phosphatidylcholine. Lipoid E80 serves as an emulsifier, stabilizer, and wetting agent in various formulations.
Automatically generated - may contain errors
Lab products found in correlation
Paraformaldehyde (pfa), by Guangzhou Chemical (3 mentions)
Quercetin, by Merck Group (2 mentions)
Pluronic f127, by BASF (2 mentions)
Ortho phenylenediamine, by Merck Group (1 mentions)
Porcine gastric mucin type 2, by Merck Group (1 mentions)
N acetylglucosamine, by Merck Group (1 mentions)
Pbs bsa solution, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Methylthiazolyldiphenyl tetrazolium bromide mtt, by Beyotime (1 mentions)
Medium chain triglycerides, by Lipoid (1 mentions)
Luteolin, by Merck Group (1 mentions)
3 o methylquercetin, by Merck Group (1 mentions)
Nile red, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Lach-Ner (1 mentions)
Metformin hydrochloride, by Merck Group (1 mentions)
Arium 611, by Sartorius (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Carl Roth (1 mentions)
Hydrochloric acid hcl, by Carl Roth (1 mentions)
6 protocols using egg lecithin e80
1
Lectin-Based Nanoparticle Targeting Mucin
2
Cytarabine-Loaded Nanoparticle Formulation
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Cytarabine (purity 99.6%, MW 279.68) was purchased from Peking University Pharmaceutical Co., Ltd. (Beijing, China). Egg lecithin (E80) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Sodium Cholesteryl sulfate (SCS, MW 488.7) was obtained from Hubei Saibo Medical Chemistry Goods Limited Company; Octadecylamine (ODA, MW 269.51) was supplied by Sigma International Inc. ε-polylysine (PLL, molecular weight 4000kDa) was purchased from Lanzhou Weiri Bioengineering Limited Company. γ-Polyglutamic acid (PGA, molecular weight 1200kDa) was obtained from Zhejiang Haining City Biotechnology Violet Gold Harbour Limited Company. Sodium sulfite was supplied by Nanjing Longyan Chemical Co., Ltd. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Beyotime Institute of Biotechnology. Fluorescein isothiocyanate (FITC) was obtained from Sigma International Inc. Paraformaldehyde was purchased from Guangzhou Chemical Reagent Factory. Chromatographic grade methanol was purchased from Concord Technology (Tianjin) Co., Inc. Water was purified by a Barnstead EASYpure® II RF/UV ultrapure water system (Dubuque, Lowa, USA). All other materials were of analytical grade.
3
Chitosan-Based Nanoparticle Formulation
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Chitosan (MW 100 kDa; approximately 90% deacetylated) was obtained from Golden-Shell Biochemical Co. Ltd. (Zhejiang, People’s Republic of China). Egg lecithin (Lipoid E80) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Pluronic F127 was a kind gift from BASF (Ludwigshafen, Germany). HPMC and hydroxyethyl cellulose (HEC) were purchased from Colorcon Co. Ltd. (Shanghai, People’s Republic of China) and Ashland-Aqualon Inc. (Wilmington, DE, USA), respectively. Negative control siRNA and Cy5-labeled siRNA (Cy5-siRNA) were purchased from Shanghai GenePharma Co. Ltd. (Shanghai, People’s Republic of China) and Biomics Biotechnologies Co. Ltd. (Jiangsu, People’s Republic of China), respectively. All other chemicals and solvents were of analytical reagent grade.
4
Achyrocline satureioides Extract Characterization
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The Achyrocline satureioides plant (voucher specimen: number 308) was obtained from CPQBA-UNICAMP (São Paulo, Brazil). Egg-lecithin (Lipoid E-80) and medium chain triglycerides (MCT) were purchased from Lipoid GmbH (Ludwigshafen, Germany). Polysorbate 80 and vitamin E were supplied from Vetec Química Fina Ltda. (Rio de Janeiro, Brazil) and Alpha Química (Porto Alegre, Brazil), respectively. Quercetin, luteolin, and 3-O-methylQuercetin standards were obtained from Sigma (Steinheim, Germany), Alfa Aesar (Lancashire, United Kingdom), and Extrasynthese (Genay, France), respectively. Nile Red was purchased from Sigma (Steinheim, Germany). Porcine ears and esophagus were gently donated by Ouro do Sul Ltda. (Harmonia, Brazil). All solvents used were HPLC grade: methanol (J.T. Baker, Center Valley, USA), acetonitrile (Tedia Brasil, Rio de Janeiro, Brazil), and phosphoric acid (Merck, Darmstadt, Germany).
5
Preparation of Phospholipid-Based Formulations
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Deionized water was obtained on Arium 611, Sartorius (Sartorius Lab Instruments GmbH & Co. KG, Göttingen, Germany). The materials used in this experimental work include: disodium hydrogen phosphate dihydrate (Kemika, Zagreb, Croatia), cholesterol (Sarajevolijek, Sarajevo, Bosnia and Herzegovina), potassium dihydrogen phosphate (Semikem, Sarajevo, Bosnia and Herzegovina), disodium hydrogen phosphate (Kemika, Zagreb, Croatia), ketoprofen, working standard (LEK, Slovenia), metformin hydrochloride, reference standard (Merck, Darmstadt, Germany), sodium chloride (Merck, Darmstadt, Germany), n-octanol (Semikem, Sarajevo, Bosnia and Herzegovina), hydrochloric acid (Lach-ner, sro Neratovice, Czech Republic), 85 % phosphoric acid (Mallinckrodt Baker BV, Deventer, The Netherlands). Egg lecithin (Lipoid® E80) was a kind gift from Lipoid GmbH, Ludwigshafen, Germany.
6
Conjugating Chitosan for Biomedical Applications
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Low-molecular-weight CS
(50–190 kDa), 6-MNA (technical grade, 90%), N-hydroxysuccinimide (NHS) (98%),l -cysteine (≥97%), l -cysteine hydrochloride monohydrate (l -cysteine-HCl
× H2O) (≥98%), 6,6′-dithiodinicotinic
acid (6,6′-DTNA) (technical grade, 85%), fluorescein isothiocyanate
(FITC) (≥90%), L-glutathione (reduced, ≥99%), 5,5′-dithiobis(2-nitrobenzoic
acid) (DTNB) (≥98%), pepsin from porcine gastric mucosa (≥250
U/mg), potassium dihydrogen phosphate (KH2PO4) (99.5–100.5%, Ph. Eur.), pancreatin from porcine pancreas
(4× USP specification), potassium chloride (KCl) (for analysis),
protease inhibitors, sodium borohydride (≥98%), and sodium
phosphate monobasic (Na2HPO4) (Ph. Eur, USP)
were purchased from Sigma-Aldrich (Vienna, Austria). Egg lecithin
(Lipoid E80) was a kind gift from Lipoid GmbH (Ludwigshafen, Germany).
Pluronic F127 and Lumogen Red (LGR) were donated from BASF (Ludwigshafen,
Germany). Sodium chloride (99.5%), N,N-dimethylformamide (DMF) (≥99%), and cetyl palmitate (98%)
were products from Acros (Geel, Belgium). Acetic acid (100%, Ph. Eur.),
1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride
(EDAC × HCl) (≥99%), dimethyl sulfoxide (DMSO) (≥99.5%),
hydrochloric acid (HCl) (37%), and sodium hydroxide (NaOH) pellets
were purchased from Roth (Karlsruhe, Germany). FaSSIF/FeSSIF/FaSSGF
powder was purchased from Biorelevant (London, U.K.).
(50–190 kDa), 6-MNA (technical grade, 90%), N-hydroxysuccinimide (NHS) (98%),
× H2O) (≥98%), 6,6′-dithiodinicotinic
acid (6,6′-DTNA) (technical grade, 85%), fluorescein isothiocyanate
(FITC) (≥90%), L-glutathione (reduced, ≥99%), 5,5′-dithiobis(2-nitrobenzoic
acid) (DTNB) (≥98%), pepsin from porcine gastric mucosa (≥250
U/mg), potassium dihydrogen phosphate (KH2PO4) (99.5–100.5%, Ph. Eur.), pancreatin from porcine pancreas
(4× USP specification), potassium chloride (KCl) (for analysis),
protease inhibitors, sodium borohydride (≥98%), and sodium
phosphate monobasic (Na2HPO4) (Ph. Eur, USP)
were purchased from Sigma-Aldrich (Vienna, Austria). Egg lecithin
(Lipoid E80) was a kind gift from Lipoid GmbH (Ludwigshafen, Germany).
Pluronic F127 and Lumogen Red (LGR) were donated from BASF (Ludwigshafen,
Germany). Sodium chloride (99.5%), N,N-dimethylformamide (DMF) (≥99%), and cetyl palmitate (98%)
were products from Acros (Geel, Belgium). Acetic acid (100%, Ph. Eur.),
1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride
(EDAC × HCl) (≥99%), dimethyl sulfoxide (DMSO) (≥99.5%),
hydrochloric acid (HCl) (37%), and sodium hydroxide (NaOH) pellets
were purchased from Roth (Karlsruhe, Germany). FaSSIF/FeSSIF/FaSSGF
powder was purchased from Biorelevant (London, U.K.).
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