Egm 2mv growth media

Manufactured by Lonza

Sourced in Switzerland

EGM™-2MV is a media formulation designed for the cultivation of mammalian cells. It provides the necessary nutrients and growth factors to support cell proliferation and viability in vitro.

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Lab products found in correlation

Hcaec, by Lonza (2 mentions) Paclitaxel, by Polysciences (2 mentions) Paclitaxel, by LC Laboratories (2 mentions) Plasmotest mycoplasma detection kit, by InvivoGen (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Hmvec d, by Lonza (2 mentions) Human hek293t, by Takara Bio (2 mentions) Polyethylenimine (pei), by Polysciences (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Hdmec, by Lonza (1 mentions) Huvecs, by Thermo Fisher Scientific (1 mentions) Egm 2, by Lonza (1 mentions) Huvecs, by Lonza (1 mentions) Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Lsm 770, by Zeiss (1 mentions)

Spelling variants of this product

EGM-2MV (246 citations) EGM-2MV media (35 citations) EGM media (12 citations) Growth media (5 citations)

7 protocols using egm 2mv growth media

Paclitaxel Delivery to Endothelial Cells

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Example 3

Delivery of paclitaxel to endothelial cells and tissue was studied in vitro using cells grown on Matrigel™ coated cell culture plates. Human coronary endothelial cells (HCAECs, Lonza, Walkersville, Md.) were cultured in EGM™-2MV growth media (Lonza, Walkersville, Md.). One day prior to paclitaxel transfer studies, cells were seed in 96 well BD Matrigel™ Matrix Thin-Layer cell culture plates at 20,000 cells per well in 0.2 mL of medium. Suspensions of paclitaxel (LC Laboratories, Woburn, Mass.) in water were prepared at 11 mg/ml paclitaxel and with PEI (Polysciences, Warrington, Pa.; MW=750 kDa) or PAMAM, ethylene diamine core, gen 4, dendrimer (Sigma, Milwaukee, Wis.; 14,214 Da) at 0.96 mg/mL (92:8 w/w ratio) or iopromide at 11 mg/mL (IOPR, 1:1 w/w ratio). Suspensions were sonicated briefly prior to use. Resulting suspensions (5 μL) were added to the cell culture plates and incubated for 3 or 10 minutes. Suspensions were also added to Matrigel™ coated plates with medium but without cells. After incubation plates were rinsed three times with phosphate buffered saline (200 μL per well) and then allowed to dry overnight. paclitaxel remaining in plates was dissolved in methanol (250 μL) and quantified by HPLC. The amount of transferred paclitaxel is shown in FIG. 3.

US09999675B2. Composition and method for delivery of hydrophobic active agents (2018-06-19). Surmodics, Inc. [US]. Inventors: Joseph Ventura [US], Shannon Wadman [US], Joram Slager [US], Joseph Schmidt McGonigle [US], Robert W. Hergenrother [US].

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Fabrication of Dual-Cell Vascular Grafts

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For cell culture studies, the nanofibrillar collagen scaffolds were sterilized in 70% ethanol and rehydrated in PBS. The collagen scaffolds were seeded with either primary human dermal microvascular ECs (HMDECs, Lonza, passage 7-10) in EGM-2MV growth media (Lonza) or primary human aortic vascular smooth muscle cells (SMCs, Life Technologies, passage 6-10) in Medium 231 with smooth muscle growth supplement (SMGM, Life Technologies). ECs and SMCs were seeded at 125,000 cells/cm² and cultured in a 1:1 ratio of endothelial growth media (EGM-2MV) media to Medium 231 with smooth muscle growth supplement (SMGM, Life Technologies) at 37°C and 5% CO². Bi-layered grafts were seeded first with SMCs that were allowed to attach to the outer layer, with nylon tubing placed in the lumen of the graft to prevent cell adhesion to the intimal layer. After incubation for 16h, the tubing was then removed and HMDECs were seeded into the inner lumen using a 20G syringe.

Nakayama K.H., Joshi P.A., Lai E.S., Gujar P., Joubert L.M., Chen B, & Huang N.F. (2015). Bilayered vascular graft derived from human induced pluripotent stem cells with biomimetic structure and function. Regenerative Medicine, 10(6), 745-755.

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Culturing Human Dermal Microvascular and HEK293T Cells

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Human dermal microvascular endothelial cells (hMVEC-Ds, Lonza) were maintained in EGM2-MV growth media (Lonza) and used at passages 2–10. Human HEK293T (Clonetech) were cultured in DMEM (Hyclone) supplemented with 10% fetal bovine serum (Hyclone), 100 U ml⁻¹ penicillin, 100 µg ml⁻¹ streptomycin (Life Technologies) and 2 mM L-glutamine (Life Technologies). Both cell types were maintained at 37 °C in 5% CO2 in a humidified incubator. Cell line authentication (performance, differentiation, and STR profiling) was provided by Lonza. was Mycoplasma testing was performed on all lines using PlasmoTest mycoplasma detection kit from InvivoGen.

Polacheck W.J., Kutys M.L., Yang J., Eyckmans J., Wu Y., Vasavada H., Hirschi K.K, & Chen C.S. (2017). A non-canonical Notch complex regulates adherens junctions and vascular barrier function. Nature, 552(7684), 258-262.

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Culturing Human Dermal Microvascular and HEK293T Cells

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Endothelial Cell Culture Protocols

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HUVECs, human dermal microvascular ECs (HDMECs), and EGM-2 and EGM-2-MV growth media were purchased from Lonza (Basel, Switzerland). Human brain microvascular ECs (HBECs) and Complete Human EC medium were purchased from Cell Biologics (Chicago, IL, USA). 293T cells were cultured in DMEM high glucose (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum and sodium pyruvate 1 mM. All our experiments were performed using HUVECs and HDMECs at passage 4 or 5.

Perrot C.Y., Sawada J, & Komatsu M. (2018). Prolonged activation of cAMP signaling leads to endothelial barrier disruption via transcriptional repression of RRAS. The FASEB Journal, 32(11), 5793-5812.

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Cytotoxicity and Cell Alignment on Collagen Scaffolds

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To assess for cytotoxicity of the scaffolds, randomly oriented or aligned collagen scaffolds were disinfected with 70% ethanol and rehydrated with phosphate-buffered saline (PBS) for 2 h. Primary human dermal microvascular endothelial cells (0.5 × 10⁶ cells, Lonza) were seeded per collagen scaffold and cultured in EGM-2 MV growth media (Lonza) at 37 °C and 5% CO2 until they reached approximately 80% confluence. After 24 h, the cell-seeded scaffolds (n = 3 each) were fixed in 4% paraformaldehyde and samples were stained for F-actin using Alexa Fluor-488-conjugated phalloidin (Life Technologies). Samples were then counterstained with Hoechst33342 to visualize nuclei and imaged in the hydrated state in PBS. Scaffolds were imaged using confocal microscopy (Zeiss LSM 770). To examine the ability of aligned nanofibrillar scaffolds to modulate cellular organization, green fluorescent protein-tagged mouse myoblasts (C2C12, ATCC) were seeded onto the scaffolds and allowed to fuse for 5 days in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 2% horse serum. Cells on the scaffolds were imaged live using confocal microscopy (Zeiss LSM 770).

Nakayama K.H., Alcazar C., Yang G., Quarta M., Paine P., Doan L., Davies A., Rando T.A, & Huang N.F. (2018). Rehabilitative exercise and spatially patterned nanofibrillar scaffolds enhance vascularization and innervation following volumetric muscle loss. NPJ Regenerative Medicine, 3, 16.

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Paclitaxel Delivery to Endothelial Cells

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Example 2

Delivery of paclitaxel to surfaces with or without seeded endothelial cells was studied in-vitro using Matrigel™ coated 96-well cell culture plates (BD Matrigel™ Matrix Thin-Layer cell, available from Becton Dickinson Biosciences, Franklin Lakes, N.J.). Human coronary endothelial cells (HCAECs, available from Lonza, Walkersville, Md.) were cultured in EGM™-2MV growth media (available from Lonza, Walkersville, Md.). One day prior to paclitaxel transfer studies, cells were seed in the various culture plates at 20,000 cells per well in 0.2 mL of medium. Suspensions of paclitaxel (LC Laboratories, Woburn, Mass.) in water were prepared at 11 mg/ml paclitaxel with or without PEI (available from Polysciences, Warrington, Pa.; MW=750 kDa) at 1 mg/ml. Suspensions were sonicated briefly prior to use. Resulting suspensions (5 μL) were added to 0.1 mL of cell media and put in the cell culture plates and incubated for 3 minutes. Suspensions were also added to the Matrigel™ coated plate with 0.1 mL medium but without seeded cells. After incubation plates were rinsed three times with phosphate buffered saline (0.2 mL per well) and then allowed to dry overnight. paclitaxel remaining in plates as a result of adhesion was dissolved in 250 μL methanol/0.1% acetic acid and quantified by HPLC. The amount of transferred paclitaxel is shown in FIG. 2.

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