Chemical compounds: Hippuryl-L-Histidyl-L-Leucine (HHL), pepstatin A, phenylmethanesulfonyl fluoride (PMSF), α-amylase from hog pancreas (50 U mg−1), pepsin from porcine gastric mucosa (250 U mg−1), pancreatin from porcine pancreas, bile extract, 2,4,6-trinitrobenzenesulfonic acid (TNBS), 3,5-dinitrosalicylic acid (DNS), p-nitrophenyl acetate (pNPA), starch solution, trypsin, penicillin, streptomycin, phosphate-buffered saline (PBS) without Ca2+ and Mg2+, hydrocortisone, sodium pyruvate, sodium bicarbonate, fetal bovine serum (FBS), resazurin and dimethyl sulfoxide (DMSO), Mueller-Hinton broth (MHB), Muller-Hinton agar (MHA )were purchased from Sigma-Aldrich (St. Louis, MO, USA). The DMEM/F12 (1:1) medium was purchased from ATCC (Manassas, VA, USA).
Dmem f12 1 1 medium
Manufactured by American Type Culture Collection
Sourced in United States
DMEM/F12 (1:1) medium is a basal medium formulation commonly used for the culture of a wide variety of cell types. It is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture, providing a balanced combination of nutrients, vitamins, and salts to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Trypsin, by Merck Group (2 mentions)
Penicillin, by Merck Group (2 mentions)
Streptomycin, by Merck Group (2 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
Hydrocortisone, by Merck Group (2 mentions)
Sodium pyruvate, by Merck Group (2 mentions)
Pepstatin a, by Merck Group (1 mentions)
P nitrophenyl acetate, by Merck Group (1 mentions)
3 5 dinitrosalicylic acid, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by American Type Culture Collection (1 mentions)
Streptomycin, by American Type Culture Collection (1 mentions)
Hct116, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Ac devd cho, by Merck Group (1 mentions)
3 protocols using dmem f12 1 1 medium
1
In Vitro Assays for Enzyme Activity
2
Culturing Colon Cancer and Normal Cell Lines
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The HCT116 and SW480 colon cancer cell lines and FHC colon epithelial normal cells were obtained from American Type Culture Collection (ATCC). HCT116 was cultured in DMEM (Gibco, Life Technologies, Grand Island, NY) medium supplemented with 10% heat inactivated fetal bovine serum (FBS) and 100 units/ml penicillin, 100 μg/ml streptomycin (Invitrogen). SW480 was cultured in RPMI 1640 medium supplemented with 10% heat inactivated FBS,100 units/ml penicillin and 100 μg/ml streptomycin. FHC was cultured in DMEM: F12 (1:1) medium (ATCC) supplemented with 10% heat inactivated FBS, 25 mM HEPES, 100 units/ml penicillin and 100 μg/ml streptomycin. Cell cultures were then maintained in an incubator within a humidified atmosphere of 5% CO2 at 37°C.
3
Evaluating Cytotoxicity and Oxidative Stress
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Trypsin, penicillin, streptomycin, neutral red solution, 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA), phosphate-buffered saline (PBS) without Ca2+ and Mg2+, hydrocortisone, Hoechst 33342, calcein AM, sodium pyruvate, sodium bicarbonate, fetal bovine serum (FBS), N-acetyl-L-cysteine (NAC), staurosporine, Ac-DEVD-CHO (caspase-3 inhibitor), hydrogen peroxide (H2O2) and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The DMEM/F12 (1:1) medium was purchased from ATCC (Manassas, VA, USA). Caspase-3 substrate (Ac-DEVD-pNA) was purchased from Calbiochem (Merck Corporation, Darmstadt, Germany). The LDH-based cytotoxicity detection kit was purchased from Roche Applied Science (Mannheim, Germany). H2DCFDA, Hoechst 33342, calcein AM and staurosporine stock solutions were prepared by dissolving the compounds in DMSO. Plant extracts were dissolved in ethanol. The final concentration of ethanol in the culture medium was always 0.1%.
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