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Quantitect sybr green qpcr

Manufactured by Qiagen
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The Quantitect Sybr Green qPCR is a reagent kit for quantitative real-time PCR (qPCR) analysis. It contains a ready-to-use master mix that includes the SYBR Green I dye, enabling the detection and quantification of DNA sequences.

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Lab products found in correlation

Quantitect reverse transcription kit, by Qiagen (1 mentions)

Close spelling variants of this product

SYBR Green (372 citations) QuantiTect (227 citations) QuantiTect SYBR Green (99 citations) Quantitect SYBR Green qPCR kit (11 citations) QuantiTect SYBR Green RT-qPCR kit (10 citations) QuantiTect SYBR Green qPCR Master Mix (8 citations) QuantiTect SYBR Green qPCR mix (3 citations) QPCR SYBR Green (2 citations) QuantiTect SYBR Green 1-step real-time RT-qPCR Kit (2 citations)

2 protocols using quantitect sybr green qpcr

1

Yeast and Mouse Gene Expression Analysis

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RNA was extracted from tissues using QIAzol and cDNA was synthesised using the Quantitect Reverse Transcription Kit (Qiagen). qPCR was conducted using Quantitect Sybr Green qPCR (Qiagen) with the following primers: PrimePCR™ SYBR® Green Assays to yeast Thr1 (qSceCED0001111, Bio-Rad) and Thr4 (qSceCED0005815, Bio-Rad) as well as mouse Tbp (QT00198443, Qiagen) as a housekeeping gene.
Yap Y.W., Rusu P.M., Chan A.Y., Fam B.C., Jungmann A., Solon-Biet S.M., Barlow C.K., Creek D.J., Huang C., Schittenhelm R.B., Morgan B., Schmoll D., Kiens B., Piper M.D., Heikenwälder M., Simpson S.J., Bröer S., Andrikopoulos S., Müller O.J, & Rose A.J. (2020). Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution. Nature Communications, 11, 2894.
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2

Quantifying Bacterial DNA in Mosquitoes

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The amount of total bacterial DNA was determined in triplicate for individual mosquitoes using QuantiTect SYBR Green qPCR (Qiagen) with the same primers utilized for 16S sequencing (V3V4 hypervariable region). A standard curve was generated by extracting DH5-Alpha Escherichia coli, quantitating DNA utilizing a NanoDrop 2000 and calculating genome copies. A 10-fold serial dilution was created from 102 to 108 genome copies.
Zink S.D., Van Slyke G.A., Palumbo M.J., Kramer L.D, & Ciota A.T. (2015). Exposure to West Nile Virus Increases Bacterial Diversity and Immune Gene Expression in Culex pipiens. Viruses, 7(10), 5619-5631.
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