Anti phospho pkcα β 2

Western blot analysis (WB) was carried out essentially as previously described in (63 (link)). The detection and quantification were performed with Odyssey Clx System (LI-COR Biosciences) through the Image Studio Software, or by traditional ECL detection. The following antibodies were used: anti-ZEB1-1642 (from Dr. Douglas Darling Lab), anti-ZEB1 (Santa Cruz,# sc-25388), anti-E-cadherin (BD Biosciences,# 610182), anti-vimentin (Cell Signaling, #5741), anti-SNAIL (Cell Signaling, #3879), anti β-catenin (Cell Signaling, #8480), anti ZO-1 (Cell Signaling, #8193), anti-cytokeratin 18 (Cell Signaling, #4548), anti-phospho serine/threonine (Abcam, #ab9337), anti-phospho-substrates antibodies kit Cell Signaling, #9920) anti-GFP (Abcam, #ab290), anti-PKCα (Santa Cruz, # sc-208), anti-PKCδ (Cell Signaling, #2058) anti-PKCε (Santa Cruz,# sc-1681), anti- Phospho-PKCα/β II (Cell Signaling, #9375) anti-α-tubulin (Sigma-Aldrich, #T5168); anti-β-actin (Sigma-Aldrich, #A2228); anti-PCNA (Santa Cruz, #sc56). As secondary antibodies we used anti-rabbit-Alexa-Fluor 594 (Molecular Probes), anti-rabbit HRP (Cell Signaling, #7074), anti-mouse HRP (Cell Signaling, #7076), goat anti-mouse IRDye 680RD and goat anti-rabbit IRDye 800CW (LI-COR Biosciences).

Diphenyleneiodonium chloride (DPI), Gö6976, U0126, SB202190, SP600125, and helenalin were obtained from Biomol (Plymouth Meeting, PA). Apocynin was from ChromaDex (Santa Ana, CA). N-acetylcysteine (NAC), tricarbonyldichlororuthenium (II) dimer (CORM-2) and ruthenium (III) chloride (inactive CORM-2) were purchased from Sigma (St. Louis, MO). Anti-GAPDH antibody was obtained from Biogenesis (Boumemouth, UK). Anti-p47^phox, anti-HO-1, anti-Gsα, anti-gp91^phox, anti-β-actin, and anti-cPLA₂ antibodies were from Santa Cruz (Santa Cruz, CA). Anti-phospho-p38 MAPK, anti-phospho-JNK1/2, anti-phospho-PKCα/βII, anti-phospho-p65, and anti-phospho-IKKα/β antibodies were from Cell Signaling (Danvers, MA). Dihydroethidium (DHE) was from Molecular Probes (Eugene, OR).