Anti hyl1

Manufactured by Agrisera

Sourced in United States

Anti-HYL1 is a laboratory product used for protein detection and analysis. It functions as an antibody that specifically binds to the HYL1 (Hyponastic Leaves 1) protein, which is involved in small RNA biogenesis in plants. This product can be used in various research applications that require the identification and quantification of HYL1 protein levels.

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Lab products found in correlation

Anti gst, by Merck Group (2 mentions) Anti flag, by Merck Group (2 mentions) Anti mbp, by New England Biolabs (1 mentions) Anti myc peroxidase, by Merck Group (1 mentions) Anti actin, by EASYBIO (1 mentions) Anti myc, by Merck Group (1 mentions) Anti rabbit igg antibody, by GE Healthcare (1 mentions) Anti lh, by New England Biolabs (1 mentions) Anti gfp, by Merck Group (1 mentions)

3 protocols using anti hyl1

Western Blot Antibody Validation

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Anti-Flag (Sigma-Aldrich, St Louis, MO, USA; F3165, 1:5000 dilution), anti-GST (Sigma-Aldrich; 1:5000 dilution), anti-MBP (NEB, 1:3000 dilution) and anti-HYL1 (Agrisera, 1:1000 dilution) antibodies were used for western blotting. Secondary antibodies were goat-developed anti-rabbit IgG (GE Healthcare; NA931V, 1:20 000 dilution) and goat-developed anti-mouse IgG (GE Healthcare; NA934V, 1:50 000 dilution).

Yang X., Ren W., Zhao Q., Zhang P., Wu F, & He Y. (2014). Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA. Nucleic Acids Research, 42(19), 12224-12236.

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Protein Extraction and Western Blotting

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The procedures used for protein extraction and western blotting have been described previously [5 ]. The following antibodies were purchased from commercial sources: anti-FLAG-peroxidase (Sigma, St. Louis, MO, USA), anti-Myc-peroxidase (Sigma), anti-Myc (Sigma), anti-FLAG (Sigma), anti-Actin (Easybio, Beijing, China), and anti-HYL1 (Agrisera AB Box 57, SE-911 21 Vännäs, Sweden).

Zheng L., Zhang C., Shi C., Yang Z., Wang Y., Zhou T., Sun F., Wang H., Zhao S., Qin Q., Qiao R., Ding Z., Wei C., Xie L., Wu J, & Li Y. (2017). Rice stripe virus NS3 protein regulates primary miRNA processing through association with the miRNA biogenesis factor OsDRB1 and facilitates virus infection in rice. PLoS Pathogens, 13(10), e1006662.

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Western Blot Antibody Validation

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Anti-GFP (Sigma-Aldrich, St Louis, MO, USA; F3165, 1:5000 dilution), anti-GST (Sigma-Aldrich; 1:5000 dilution), anti-LH (NEB, 1:3000 dilution) and anti-HYL1 (Agrisera, 1:1000 dilution) antibodies were used for Western blotting. The secondary antibodies used were goat-developed anti-rabbit IgG antibodies (GE Healthcare; NA931V, 1:20 000 dilution).

Ren W., Wu F., Bai J., Li X., Yang X., Xue W., Liu H, & He Y. (2020). BcpLH organizes a specific subset of microRNAs to form a leafy head in Chinese cabbage (Brassica rapa ssp. pekinensis). Horticulture Research, 7, 1.

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