Microplate reader at 570 nm

Manufactured by Agilent Technologies

Sourced in United States

The Microplate reader at 570 nm is a laboratory instrument designed to measure the absorbance of samples in a microplate format. It is capable of detecting and quantifying the light absorption of samples at a wavelength of 570 nanometers.

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Lab products found in correlation

Sw1353, by American Type Culture Collection (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Cell growth determination kit, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Human il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Prism 8, by GraphPad (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Sybr green master mix kit, by Roche (1 mentions)

Close spelling variants of this product

Microplate reader (6925 citations) Microplate reader at 570 (3 citations)

11 protocols using microplate reader at 570 nm

Chondrosarcoma Cell Line SW1353 Viability Assay

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The human chondrosarcoma cell line SW1353 from American Type Culture Collection (Manassas, VA, USA). The cells were cultured in DMEM supplemented with 10% FBS and 1% Penicillin-streptomycin at 37 °C and 5% CO₂ in a humidified incubator.
SW1353 cells, density of 1 × 10⁴ cells/well, were seeded in a 96-well plate followed by incubation for 24 h. After 24 h, the cells were treated with different concentrations of WG for an additional 24 and 48 h. Media were changed to media with MTT dye, and the cells were incubated for another 4 h. After the supernatants were removed, 100 μL of DMSO were added to the cells to dissolve the formazan. Finally, the plate was gently agitated on a shaker, and the absorbance was measured with a microplate reader at 570 nm (Biotek, VT, USA).

Ali A., Park Y., Lee J., An H.J., Jin J.S., Lee J.H., Chang J., Kim D.K., Goo B., Park Y.C., Leem K.H., Seong S, & Kim W. (2021). In Vitro Study of Licorice on IL-1β-Induced Chondrocytes and In Silico Approach for Osteoarthritis. Pharmaceuticals, 14(12), 1337.

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Crystal Violet Assay for Cell Viability

Cited 2 times

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Cell viability was assayed using the crystal violet method as described (12 (link),13 (link),28 (link)). Subconfluent HUVECs were seeded in 96-well plates (10,000 cells/well). After 16 hr of culture, cells were treated with 1.25, 2.5, 5, 10, or 20 μM of Kyn (Cat #: K8625) or ILA (Cat #: I5508) in ECMb supplemented with 0.2% heated inactivated FBS and 1% p/s up to 2 days (5 wells/treatment). Stock solutions of Kyn and ILA were prepared in dimethyl sulfoxide (DMSO, Cat # D2650). Additional cells were treated with the vehicle control (DMSO, 0.02% v/v; the maximum concentration in the final Kyn and ILA solutions). Kyn, ILA, and DMSO were all purchased from Sigma-Aldrich, St. Louis, MO, USA. Media were changed with ECMb containing Kyn, ILA, or DMSO daily. At the end of treatment, the optical density value of each well was measured using a microplate reader at 570 nm (Biotek, Winooski, VT, USA).

Zhao Y.J., Zhou C., Wei Y.Y., Li H.H., Lei W., Boeldt D.S., Wang K, & Zheng J. (2021). Differential Distribution of Tryptophan-Metabolites in Fetal and Maternal Circulations during Normotensive and Preeclamptic Pregnancies. Reproductive sciences (Thousand Oaks, Calif.), 29(4), 1278-1286.

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Evaluating Ps-GOS Cell Viability

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To examine effect of Ps-GOS on cell viability, RAW 264.7 cells at a density of 5 × 10³ cells/well were seeded onto a 96-well plate. After cell attachment (overnight incubation), the cells were then incubated with various doses of Ps-GOS (0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10, 100 and 1000 µg/mL) for 24, 48 and 72 h. After incubation, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. Then, formazan was dissolved in 50 mL of dimethyl sulphoxide (DMSO). Finally, we detected the optical density (OD) of each well using a microplate reader at 570 nm (Bio-tek Instruments, Winooski, VT, USA).

Rattajak P., Aroonkesorn A., Smythe C., Wititsuwannakul R, & Pitakpornpreecha T. (2024). Pleurotus sajor-caju (Fr.) Singer β-1,3-Glucanoligosaccharide (Ps-GOS) Suppresses RANKL-Induced Osteoclast Differentiation and Function in Pre-Osteoclastic RAW 264.7 Cells by Inhibiting the RANK/NFκB/cFOS/NFATc1 Signalling Pathway. Molecules, 29(9), 2113.

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MTT Assay for Cell Viability

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Cells were tested for vitality using the MTT assay as previously described [31 (link)]. The cells were plated at 2.0 × 10⁵ cells/mL in 96-well plates and supplemented with CO at various concentrations (0.125, 0.25, 0.5, 1, and 2%) or celecoxib (CX) at 10 µM for 24 h. Afterward, cells were treated for four hours at 37 °C with the MTT reagent and then added to the DMSO solution. Cell viability was measured using a microplate reader at 570 nm (BioTek, Winooski, VT, USA).

Ngernjan M., Ontawong A., Lailerd N., Mengamphan K., Sarapirom S, & Amornlerdpison D. (2022). Crocodile Oil Modulates Inflammation and Immune Responses in LPS-Stimulated RAW 264.7 Macrophages. Molecules, 27(12), 3784.

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MTT Assay for Cell Viability

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Cell Growth Determination Kit (Sigma, USA) was used for this assay in accordance with the
manufacturer’s protocol. The cells were seeded at a density of 4×10³ cells per
well and incubated at 37°C in 5% CO₂ . For seven consecutive days, we added 50
µl MTT solution [5 mg/ ml in DMEM (Invitrogen, USA)] to the cells seeded in each well. The
plates were incubated at 37°C for 4 hours to enable conversion of MTT to formazan crystals
by mitochondrial dehydrogenases in the viable cells. The supernatant was removed for
dissolution of dark blue intracellular Formazan, and a constant amount of dimethyl
sulfoxide solvent was added. Optical density was read in a microplate reader at 570 nm
(BioTek Instruments, Winooski, VT, USA). Finally, the cell numbers were calculated using a
standard curve.

Naseri Mobaraki S., Abroun S., Atashi A, & Kaviani S. (2023). Evaluation of Expansion and Maintenance of Umbilical Cord Blood CD34+ Cells in The Co-Culture with Umbilical Cord Blood-Derived Mesenchymal Stem Cells in The Presence of Microcarrier Beads. Cell Journal (Yakhteh), 25(3), 184-193.

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Quantification of IL-6 in Cell Cultures

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Concentration of IL-6 in cell culture supernatants was determined using a human IL-6 ELISA kit of Thermo Fisher Scientific Inc (Madrid, Spain) according to manufacturer's instructions. The ELISA 96-well micro plates were analysed using a microplate reader at 570 nm (Bio-Tek, Winooski, VT, USA).

Álvarez-Cilleros D., López-Oliva M.E., Ramos S, & Martín M.Á. (2020). Preventive effect of cocoa flavanols against glucotoxicity-induced vascular inflammation in the arteria of diabetic rats and on the inflammatory process in TNF-α-stimulated endothelial cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 146.

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In Vitro Antiproliferative Activity Assay

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MHCC97H were purchased from Kangyuan Bochuang Biotechnology co. LTD (Beijing, China) and cultured with DMEM medium containing 10% FBS. The in vitro antiproliferative activity of each compound was assayed using MTT method. In brief, cells were seeded in 96-well plates, at the density of 5 × 10³/well. After medium removal, 100 μL of culture media with 0.1% DMSO containing each compound at different concentrations was added to each well, and incubation continued for another 48 h at 37 °C. DMSO (0.1%) and tepotinib were used as the negative and positive controls, respectively. MTT solution (5 mg/mL in PBS) was added, and incubation continued for another 4 h. The optical density was detected with a microplate reader at 570 nm (BioTek, Winooski, VT, USA). The IC₅₀ value of each compound was calculated using GraphPad Prism 8. All the experiments were repeated independently at least three times.

Yao H., Ren Y., Yan J., Liu J., Hu J., Yan M, & Li X. (2022). Design, Synthesis, and Evaluation of New Mesenchymal–Epithelial Transition Factor (c-Met) Kinase Inhibitors with Dual Chiral Centers. Molecules, 27(17), 5359.

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Piplartine Modulates LPS-Induced Cell Viability

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Cells were seeded in 96-well plates (1 × 10⁵ cells/mL) overnight. Afterward, cells were pretreated with different concentrations (0–10 μΜ) of the piplartine (obtained from ChemFaces, Wuhan, Hunan, China) for 1 h, and then primed with 1 μg/mL LPS (from E. coli. O111:B4, purchased from Sigma Aldrich) for 24 h. MTT (5 mg/mL, purchased from Sigma Aldrich) was added to each well, and the plates were incubated at 37 °C for 4 h. The formazan crystals were lysed with 0.04 M HCl/isopropanol. The absorbance was measured by a microplate reader at 570 nm (BioTek Instruments, Winooski, VT, USA).

Huang C.H., Wang S.C., Chen I.C., Chen Y.T., Liu P.L., Fang S.H., Huang S.P., Yeh H.C., Liu C.C., Lee P.Y., Lin T.C., Cheng W.C., Su C.C., Wu H.E., Chen Y.R, & Li C.Y. (2021). Protective Effect of Piplartine against LPS-Induced Sepsis through Attenuating the MAPKs/NF-κB Signaling Pathway and NLRP3 Inflammasome Activation. Pharmaceuticals, 14(6), 588.

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Lithium Chloride Cytotoxicity Assay in PC-3 Cells

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. PC-3 was placed with 1x10 4 cells/well. Lithium chlorides (Licl) have different concentrations at various time intervals (12, 24 and 48 h). Next, each well was added with MTT solution. Then, each well was put into dimethyl sulfoxide (DMSO) and rotated at room temperature (RT). The absorbance value was measured with microplate reader at 570 nm (BioTek Instruments, Inc., Winooski, VT, USA).
RT-qPCR and RT-PCR assay. EasyPure ® kit (TransGen Biotech, Inc., Beijing, China) was applied to extract total RNA. We applied TransScript ® SuperMix (TransGen Biotech) to synthesis of cDNA. RT-qPCR was used with SYBR-Green Master Mix kit (Roche, Basel, Switzerland) and GADPH was used as the internal reference. Samples were denaturized at 95˚C for 10 min, followed by 45 cycles at 95˚C for 15 sec, 60˚C for 30 sec and 72˚C for 15 sec. cDNA template was amplified with 2X EasyTaq ® PCR Mix (TransGen Biotech) for RT-PCR. Reaction conditions were 94˚C for 5 min, 35 cycles of: 94˚C for 30 sec, 60˚C for 30 sec and 72˚C for 60 sec and 72˚C for 10 min. Then, samples were electrophoresed with 2.5% agarose gel. The primers (Sangon Biotech Co., Ltd., Shanghai, China) were designed as showen in Table I. Each sample was run in triplicate.
Table I. Primer for RT-PCR and RT-qPCR.

Liu Y., Qin Z., Yang K., Liu R, & Xu Y. (2017). Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling. Oncology reports, 37(3).

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Cell Viability Assessment on Scaffolds

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The survival of the cells was checked for 5 days of cell culture on a scaffold in the presence of basal medium. Sterilized PEO/PCL and PEO/Dex-PCL scaffolds were placed in 96-well culture plate, seeded with 5 Â 10 3 cells per cm 2 , and incubated at 37 C in 5% CO 2 . Over 1, 2, 3, 4, and 5 days cell seeding, 10 ll MTT solution (5 mg/ml in DMEM) was added to each well (n ¼ 3). For conversion of MTT to formazan crystals by mitochondrial dehydrogenases of living cells, each plate was incubated at 37 C for 3.5 h. For dissolution of dark blue intracellular formazan, the supernatant was removed and a constant amount of DMSO was added as solvent. Optical density was read in a micro-plate reader at 570 nm (BioTek Instruments, Winooski, VT. The same procedure was performed in cultured cells with TCPS as a control.

Ghiaee A., Pournaqi F., Vakilian S., Mohammadi-Sangcheshmeh A, & Ardeshirylajimi A. (2017). Adapted dexamethasone delivery polyethylene oxide and poly(ɛ-caprolactone) construct promote mesenchymal stem cells chondrogenesis. Artificial cells, nanomedicine, and biotechnology, 45(8).

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