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Ab84125

Manufactured by Abcam
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Ab84125 is a laboratory equipment product. It is a piece of lab equipment designed for a specific purpose, but the core function of this product is not detailed in the information provided. A more detailed and unbiased description cannot be given without making unsupported claims.

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Lab products found in correlation

Ab16667, by Abcam (2 mentions) Ab28346, by Abcam (2 mentions) Ab71204, by Abcam (2 mentions) Ab9512, by Abcam (2 mentions) Ab58207, by Abcam (2 mentions) Ab5690, by Abcam (2 mentions) Ab183685, by Abcam (2 mentions) Ab22378, by Abcam (2 mentions) Ab6640, by Abcam (2 mentions) Ab155101, by Abcam (2 mentions) Sag4318, by Fujifilm (2 mentions) Mbs767644, by MyBioSource (2 mentions) Sc 18355, by Santa Cruz Biotechnology (2 mentions) Pa3030a, by Thermo Fisher Scientific (2 mentions) Ne1023, by Merck Group (2 mentions) N4142, by Merck Group (2 mentions) Nogo a, by Merck Group (2 mentions) Ab22884, by Abcam (2 mentions) Ab17901, by Abcam (2 mentions) Ab60229, by Abcam (2 mentions)

5 protocols using ab84125

1

Immunohistochemical Profiling of Neural and Immune Markers

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The method has been described previously (29 (link)). Primary antibodies were to the following proteins: PDGFRα (1:200, rat, 558774, BD Biosciences), PDGFRα (1:8000, rabbit, gift from W. Stallcup, Sanford Burnham Prebys), Olig2 (1:2000, rabbit, gift from C.D. Stiles, Harvard), CD31 (1:200, rat, 553370, BD Biosciences), CD31 (1:200, rabbit, ab28346, Abcam), RNF43 (1:200, rabbit, ab84125, Abcam), WIF1 (1:200, rabbit, ab71204, Abcam), Caspase-3 (1:200, rabbit, 9661, Cell signaling), Aqp4 (1:100, mouse, ab9512, Abcam), PLVAP (1:100, rat, 553849, BD Pharmingen), Claudin5 (1:200, mouse, 352588, ThermoFisher), Fibrinogen (1:200, mouse, ab58207, Abcam), Iba1 (1:500, rabbit, SAG4318, Wako), CD11c (1:500, rabbit, MBS767644, MyBioSource), Arg1 (1:100, goat, sc-18355, Santa Cruz), iNOS (1:200, rabbit, PA3030A, ThermoFisher), CD3 (1:100, rabbit, ab5690, Abcam), CD4 (1:200, rabbit, ab183685, Abcam), CD8 (1:100, rat, ab22378, Abcam), F4/80 (1:500, rat, ab6640, Abcam), Ki67 (1:200, rabbit, ab16667, Abcam), SMI32 (1:1000, mouse, NE1023, Millipore), NF200 (1:1000, rabbit, N4142, Sigma), Wif1 (1:1000, rabbit, ab155101, Abcam).
Niu J., Tsai H.H., Hoi K.K., Huang N., Yu G., Kim K., Baranzini S.E., Xiao L., Chan J.R, & Fancy S.P. (2019). Aberrant oligodendroglial-vascular interactions disrupt the Blood Brain Barrier triggering CNS inflammation. Nature neuroscience, 22(5), 709-718.
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2

Immunohistochemical Profiling of Neural and Immune Markers

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The method has been described previously (29 (link)). Primary antibodies were to the following proteins: PDGFRα (1:200, rat, 558774, BD Biosciences), PDGFRα (1:8000, rabbit, gift from W. Stallcup, Sanford Burnham Prebys), Olig2 (1:2000, rabbit, gift from C.D. Stiles, Harvard), CD31 (1:200, rat, 553370, BD Biosciences), CD31 (1:200, rabbit, ab28346, Abcam), RNF43 (1:200, rabbit, ab84125, Abcam), WIF1 (1:200, rabbit, ab71204, Abcam), Caspase-3 (1:200, rabbit, 9661, Cell signaling), Aqp4 (1:100, mouse, ab9512, Abcam), PLVAP (1:100, rat, 553849, BD Pharmingen), Claudin5 (1:200, mouse, 352588, ThermoFisher), Fibrinogen (1:200, mouse, ab58207, Abcam), Iba1 (1:500, rabbit, SAG4318, Wako), CD11c (1:500, rabbit, MBS767644, MyBioSource), Arg1 (1:100, goat, sc-18355, Santa Cruz), iNOS (1:200, rabbit, PA3030A, ThermoFisher), CD3 (1:100, rabbit, ab5690, Abcam), CD4 (1:200, rabbit, ab183685, Abcam), CD8 (1:100, rat, ab22378, Abcam), F4/80 (1:500, rat, ab6640, Abcam), Ki67 (1:200, rabbit, ab16667, Abcam), SMI32 (1:1000, mouse, NE1023, Millipore), NF200 (1:1000, rabbit, N4142, Sigma), Wif1 (1:1000, rabbit, ab155101, Abcam).
Niu J., Tsai H.H., Hoi K.K., Huang N., Yu G., Kim K., Baranzini S.E., Xiao L., Chan J.R, & Fancy S.P. (2019). Aberrant oligodendroglial-vascular interactions disrupt the Blood Brain Barrier triggering CNS inflammation. Nature neuroscience, 22(5), 709-718.
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3

Investigating Protein Signaling in ccRCC

Cited 1 time
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Western blot assays were performed as described in our previous study [17 (link)]. Briefly, cultured cells or ccRCC tissues were lysed in RIPA lysis buffer, and protein concentrations were determined using the BCA assay kit according to the manufacturer’s protocols (Thermo Fisher Scientific, Waltham, MA, USA). The nuclear and cytoplasmic proteins from ccRCC cells were extracted using an NE-PER Nuclear and Cytoplasmic Extraction Kit (#78899; Thermo Scientific). The primary antibodies used were rabbit anti-RNF43 antibody (ab84125; Abcam), rabbit anti-LATS1 + LATS2 antibody (ab70565), rabbit anti-LATS1 + LATS2 (phospho T1079 + T1041) antibody (ab111344), rabbit anti-YAP antibody (ab52771), rabbit anti-YAP (phospho S127) antibody (ab76252), rabbit anti-GAPDH antibody (#5174), rabbit anti-β-actin antibody (#58169), and rabbit anti-histone H3 antibody (#4499), all of which were from Cell Signaling Technology (Danvers, MA, USA).
ZHU D., ZHANG L., SHI X., GAO S., YUE C., ZHANG L., BAI Y., WANG Q., OKADA A., YASUI T., WANG C., CUI X, & ZUO L. (2022). RNF43 is a novel tumor-suppressor and prognostic indicator in clear cell renal cell carcinoma. Oncology Research, 29(3), 159-174.
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4

Protein Expression Profiling in Cellular Samples

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The primary antibodies used were as follows: APC (1:100 mouse monoclonal OP80, Calbiochem), Lef1 (1:200 Rabbit mAb C12A5, Cell Signaling), anti-human Lef1 (1:100 rabbit polyclonal Ab22884, Abcam), RNF43 (1:200 rabbit polyclonal Ab84125, Abcam), ETS2 (1:200 chicken polyclonal Ab17901, Abcam), DUSP4 (1:200 rabbit polyclonal Ab60229, Abcam), SP5 (1:200 rabbit polyclonal Ab36593, Abcam), Tcf4 (1:100 mouse monoclonal 6H5-3, Upstate), Olig2 (1:3000 rabbit polyclonal from CD Stiles, Harvard), Nkx2.2 (1:100 mouse monoclonal, Developmental Studies Hybridoma Bank), PDGFRα (1:200 rat 558774, BD Biosciences), NOGO-A (1:200 rabbit, Millipore), and GFAP (1:500 mouse, Sigma).
Fancy S.P., Harrington E.P., Baranzini S.E., Silbereis J.C., Shiow L.R., Yuen T.J., Huang E.J., Lomvardas S, & Rowitch D.H. (2014). Parallel states of pathological Wnt signaling in neonatal brain injury and colon cancer. Nature neuroscience, 17(4), 506-512.
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5

Protein Expression Profiling in Cellular Samples

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The primary antibodies used were as follows: APC (1:100 mouse monoclonal OP80, Calbiochem), Lef1 (1:200 Rabbit mAb C12A5, Cell Signaling), anti-human Lef1 (1:100 rabbit polyclonal Ab22884, Abcam), RNF43 (1:200 rabbit polyclonal Ab84125, Abcam), ETS2 (1:200 chicken polyclonal Ab17901, Abcam), DUSP4 (1:200 rabbit polyclonal Ab60229, Abcam), SP5 (1:200 rabbit polyclonal Ab36593, Abcam), Tcf4 (1:100 mouse monoclonal 6H5-3, Upstate), Olig2 (1:3000 rabbit polyclonal from CD Stiles, Harvard), Nkx2.2 (1:100 mouse monoclonal, Developmental Studies Hybridoma Bank), PDGFRα (1:200 rat 558774, BD Biosciences), NOGO-A (1:200 rabbit, Millipore), and GFAP (1:500 mouse, Sigma).
Fancy S.P., Harrington E.P., Baranzini S.E., Silbereis J.C., Shiow L.R., Yuen T.J., Huang E.J., Lomvardas S, & Rowitch D.H. (2014). Parallel states of pathological Wnt signaling in neonatal brain injury and colon cancer. Nature neuroscience, 17(4), 506-512.
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