Urease (Millipore Sigma, Oakville, ON, Canada) suspensions of approximately 40 mg per 250 µL of 18.2 MΩ deionized water (Elga PURELAB flex 3 system, VWR International, Edmonton, AB, Canada) were prepared on the day of derivatization. HPLC Grade Methanol (>99.9%) was purchased from Millipore-Sigma Canada, HPLC Grade Toluene (Millipore Sigma, Oakville, ON, Canada), was dried with anhydrous sodium sulfate (Millipore Sigma, Oakville, ON, Canada) prior to use. Methoxyamine hydrochloride (Millipore Sigma, Oakville, ON, Canada) was dissolved in HPLC Grade Pyridine (Millipore Sigma, Oakville, ON, Canada) to make a 20 mg/mL solution. Also, 1 mL ampoules of N-Methyl-N-trimethylsilyltrifluoroacetamide + 1% chlorotrimethylsilane (MSTFA + 1% TMCS) were purchased from Fisher Scientific, Ottawa, ON, Canada. A 100 µg/mL solution of 4-13C methylmalonic acid was prepared in deionized water to use as an internal standard.
Anhydrous sodium sulfate
Manufactured by Merck Group
Sourced in United States, Germany, Italy, United Kingdom, Switzerland, India, Canada, Sao Tome and Principe, France
Anhydrous sodium sulfate is a chemical compound with the formula Na2SO4. It is a white crystalline solid that is commonly used as a drying agent in organic synthesis and other laboratory applications. Anhydrous sodium sulfate is highly hygroscopic, meaning it readily absorbs water from the environment, making it useful for removing traces of water from organic solvents and other materials.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (32 mentions)
Sodium chloride (nacl), by Merck Group (25 mentions)
Chloroform, by Merck Group (21 mentions)
Sodium hydroxide (naoh), by Merck Group (21 mentions)
Dichloromethane, by Merck Group (21 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (21 mentions)
N hexane, by Merck Group (18 mentions)
Ethanol, by Merck Group (17 mentions)
Sodium carbonate, by Merck Group (16 mentions)
Hydrochloric acid (hcl), by Merck Group (15 mentions)
Hexane, by Merck Group (14 mentions)
Gallic acid, by Merck Group (13 mentions)
Acetonitrile, by Merck Group (13 mentions)
Ethyl acetate, by Merck Group (12 mentions)
Diethyl ether, by Merck Group (12 mentions)
Potassium hydroxide, by Merck Group (11 mentions)
Pyridine, by Merck Group (10 mentions)
Chloroform, by Thermo Fisher Scientific (9 mentions)
Formic acid, by Merck Group (9 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (9 mentions)
204 protocols using anhydrous sodium sulfate
1
Urease Derivatization for GC-MS Analysis
2
Metabolite Extraction and Derivatization
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Methanol (>99.9%, HPLC grade, Millipore-Sigma, Oakville, ON, Canada) and ultrapure 18.2 MΩ deionized water, supplied from an Elga PURELAB Flex 3 system (VWR International, Edmonton, AB, Canada), were mixed to prepare an 80% Methanol (v/v) extraction solvent. Toluene (>99.5%, ACS grade), pyridine (>99.9%, HPLC grade), anhydrous sodium sulfate (>99.0% ACS grade), and methoxyamine hydrochloride (98%) were obtained from Millipore-Sigma (Oakville, ON, Canada). N-Methyl-N-(trimethylsilyl) trifluoroacetamide + 1% chlorotrimethylsilane (MSTFA + 1% TMCS), purchased from Fisher Scientific (Ottawa, ON, Canada), was used as a derivatizing reagent. A total of 1 mg of 13C4 methylmalonic acid (Millipore-Sigma, Oakville, ON, Canada) was dissolved in 10 mL of deionized water to prepare an internal standard solution.
3
Fatty Acid Analysis Protocol
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Nonfat dry milk (USDA organic grade A) was from Organic Valley (La Farge, WI); MRS broth was obtained from BD-Difco Laboratories (Sparks, MD); linoleic acid, phosphatidylcholine, methanolic hydrochloric acid, chloroform, methanol, anhydrous sodium sulfate, NaCl, KCl, CaCl 2 , NaHCO 3 , lysozyme, pepsin, bile salts (Ox-bile), and pancreatin were obtained from Sigma Chemicals Co. (St. Louis, MO). Methyl 9(Z),11(E)-octadecadienoate and methyl 10(E),12(Z)octadecadienoate analytical standards were from Matreya LLC (Pleasant Gap, PA).
4
Atorvastatin Pharmacokinetics and Biomarkers
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QS (Lot No. 20170504) was provided by Beijing Xiusheng Pharmaceutical Co. Atorvastatin calcium tablets (Pfizer Pharmaceuticals Ltd., Lot No. S56741), IL-2 (Lot No. R20210328), IL-6 (Lot No. R20210328), TNF-α (Lot No. R20210328), creatine kinase isozyme cardiac type (CK-MB) (Lot No. R20210327), and cardiac troponin I (cTnI) (Lot No. R20210327) kits were purchased from the Nanjing Jiancheng Institute of Biological Engineering. Sodium pentobarbital (2%), lidocaine, sodium chloride (0.9%), sodium penicillin, methoxyamine HCl, fatty acid methyl ester (C7-C30, FAMEs) standards, pyridine, and anhydrous sodium sulfate were obtained from Sigma-Aldrich (St. Louis, MO, USA). MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamide) with 1% (vol/vol) trimethylchlorosilane (MSTFA, with 1% TMCS), methanol (Optima LC-MS), acetonitrile (Optima LC-MS), hexane, dichloromethane, chloroform, and acetone were purchased from Thermo-Fisher Scientific (FairLawn, NJ, USA). Ultrapure water was produced by a Mill-Q Reference system equipped with an LC-MS Pak filter (Millipore, Billerica, MA).
5
Synthesis and Characterization of Titanium Nitrate Polymer Composites
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Titanium (IV) nitrate tetrahydrate (Ti(NO3)4.H2O, ≥99.9%), pure grade of polyethylene glycol (PEG), analytical-grade acetone (anhydrous, ≥99.0%), ethanol (96%), 3% of glutaraldehyde in phosphate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) rhodamine 123 dye, acridine orange/ethidium bromide (AO/EtBr) dye, dichloro dihydro fluorescein diacetate (DCFH-DA), and anhydrous sodium sulfate were acquired from Sigma-Aldrich ( Hamburg, Germany). Ciprofloxacin (5 μg/disc, MASTDISCS™, Mast Diagnostics Ltd.), vancomycin (Glaxo SmithKline Pharmaceuticals Ltd.), and gentamycin (Alpha Laboratories Ltd.) were purchased from local drug stores.
6
Extraction and Isolation of Hydrophobic Metabolites
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Hydrophobic metabolites (crude extract) were collected from 10 mL aliquots of 1B-23 liquid cultures using a liquid-liquid extraction technique. The 1B-23 cultures were washed twice with ≥99.7% ethyl acetate (Sigma-Aldrich, Mississauga, ON, Canada), followed by collection of the organic phase and subsequent drying with 99% anhydrous sodium sulfate (Sigma-Aldrich, Mississauga, ON, Canada). The ethyl acetate was partly dried off using an IKA® RV10™ rotary evaporator (180 mBar and 125 rpm), followed by nitrogen gas to completion. The crude extract was then suspended in 100% methanol (Sigma-Aldrich, Mississauga, ON, Canada) to a final concentration of 10 mg/mL.
7
Steam Distillation of Peppermint Essential Oil
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The PStEO was obtained from the fresh flowers using the steam distillation method using a Craveiro-type apparatus following the procedure previously described by Jianu et al. [23 (link)]. The steam and vaporized oil were condensed into liquid form by a condenser and collected in a water-cooled oil receiver to decrease the formation of artifacts due to overheating [24 (link),25 (link)]. Afterward, the PStEO was recovered from the receiver with a sterile syringe, dried on anhydrous sodium sulfate (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany), and kept at −18 °C in sealed vials for future investigation (yielding 0.013% v/w).
8
Extraction and Analysis of Natural Food Colorant
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The natural food colorant Na-Cu-Chlorophyllin (Na-Cu-Chl) was obtained from Sigma Aldrich, Bangalore. All other reagents such as acetone, ethyl acetate, methanol, ethanol, acetonitrile and water were of analytical grade. Metal salts such as zinc chloride, nickel sulfate and cobalt sulphate were obtained from SD fine chemicals while Ag 2 SO 4 , PbSO 4 , FeSO 4 , SnCl 2 and anhydrous sodium sulfate were obtained from Sigma Aldrich. All samples were filtered through a 0.45 µm nylon membrane filter papers (Supelco, Bellefonte, PA, USA) before analysis.
9
Fatty Acid Saponification and Methylation
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The oils obtained from the hexane extract were independently saponified according to the technique of Bombón and Albuja (2014) . Sodium hydroxide, concentrated hydrochloric acid, anhydrous sodium sulfate (Sigma-Aldrich), ethanol, and diethyl ether (Merck) were used. For the methylation of the fatty acids, a methanol/oil molar ratio of 11.66: 1 was used. The mixture was stirred at a temperature of 35° C for 3 minutes (Salimon et al. 2014) (link).
10
Extraction and Quantification of Squalene
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Ethyl alcohol, ammonia, diethyl ether, hexane, acetonitrile, isopropyl alcohol, anhydrous sodium sulfate, and squalene standard were obtained from Sigma Aldrich (St. Louis, MO, USA).
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