Four-week-old WT mice were fed a GuD for four weeks and then divided into four groups (n = 4–6 per group). The rIL-18 intervention procedure was adopted from the studies by Seregin SS et al.76 (link) and Levy M et al.22 (link) From days 29 to 31, two groups received either recombinant mouse interleukin-18 (rIL-18, 1 µg/mouse; BioLegend), and another two groups received vehicle (veh.) intraperitoneally (i.p.). After 2 days of last injection, experimental groups were either switched to DSS (1.4% w/v) containing water (GuD+ veh. + DSS and GuD+ rIL-18+ DSS) or continued on regular water (GuD+ veh.+ water and GuD+ rIL-18+ water). After 7 days of DSS administration, mice were euthanized to collect samples for analyzing colitis markers. Mice were maintained on the GuD throughout the study.
Ril 18
Manufactured by BioLegend
RIL-18 is a laboratory equipment product. It is designed for cell culture applications. The core function of RIL-18 is to provide a regulated incubation environment for cells.
Automatically generated - may contain errors
2 protocols using ril 18
1
Dietary Modulation of Colitis Progression
2
Splenic CD8 T Cell Activation and Proliferation
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Splenic CD8 T cells were magnetically enriched using an AutoMACS pro (Miltenyi Biotec) to >95% purity per manufacturer's instructions. The cells were fluorescein-labeled with 5 μM carboxyfluorescein succinimidyl ester (CFSE) at 37° for 10', washed 3x with RPMI-1640 medium supplemented with 2-mercaptoethanol, L-glutamine, pyruvate, non-essential amino acids, and 10% fetal bovine serum (abbreviated as RP10 in the text). The cells were cultured at 2 × 105 cells/ml at a 1:1 ratio with α-CD3/α-CD28 immobilized on beads (Miltenyi), in the presence of combinations of 10 U/ml rIL-2, 10 ng/ml rIL-12 (Biolegend), and 10 ng/ml rIL-18 (Biolegend), as indicated, for up to 3 days. On each day of analysis, cells were stained for viability (LIVE/DEAD reagent, Invitrogen), as well as with surface and/or intracellular antibodies.
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