Immulite 2000 immunoassay system

Manufactured by Siemens

Sourced in Germany, United States, United Kingdom, Belgium

The Immulite 2000 Immunoassay System is a laboratory instrument designed for automated immunoassay testing. It is capable of performing a variety of immunoassay tests, including those for hormones, therapeutic drugs, and other analytes. The system is intended to provide accurate and reliable results for clinical laboratories.

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Lab products found in correlation

Meso scale discovery sector system, by Mesoscale (2 mentions) Cobas 8000, by Roche (2 mentions) Cobas 8000 modular analyzer series, by Roche (2 mentions) Immulite 1000, by Siemens (2 mentions) Advia centaur xpt immunoassay system, by Siemens (2 mentions) Abx pentra 400, by Horiba (1 mentions) Cobas integra 400 plus, by Roche (1 mentions) Cobas e411 analyser, by Roche (1 mentions) Advia 1800 clinical chemistry system, by Siemens (1 mentions) Delphi a, by Hologic (1 mentions) Cobas 6000 analyser, by Roche (1 mentions) Advia centaur intact pth assay, by Siemens (1 mentions) Advia 2400 chemistry system, by Siemens (1 mentions) Dimension vista 1500, by Siemens (1 mentions) Acl top 550, by Werfen (1 mentions) Advia 2120 hematology system, by Siemens (1 mentions) Variant 2 turbo, by Bio-Rad (1 mentions) Insulin elisa, by Mercodia (1 mentions) Unicel dxc 800, by Beckman Coulter (1 mentions) Vitros dt60 2, by Ortho Clinical Diagnostics (1 mentions)

Close spelling variants of this product

Immulite 2000 (453 citations) Immulite 2000 system (45 citations) IMMULITE 2000 XPi Immunoassay System (30 citations) Immulite system (11 citations) Immulite Immunoassay System (8 citations)

59 protocols using immulite 2000 immunoassay system

Evaluation of IGF-I Assay Methods

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Example 7

Samples from 100 patients were split and analyzed with the LC-MS method described above. Portions of the samples were also assayed using the Siemens IMMULITE 2000 immunoassay system (Siemens Healthcare Diagnostics, Inc.), the Meso Scale Discovery SECTOR system (Meso Scale Discovery), and RIA methods (conducted by Esoterix, Inc., Test Code 500282, Blocking RIA after acid:alcohol extraction). Of the 100 split samples, 60 were analyzed with the IMMULITE system.

Data from the four methods were analyzed by Deming regression. Results of the comparisons are shown in Table 7. The LC-MS analysis was demonstrated to have the best agreement with the RIA method.

TABLE 7

Deming regression analysis of comparison

of four intact IGF-I assay methods

MethodsVariable

ComparednmbSy · x

LC-MS vs RIA100 1.039 ± 0.01572−11.55 ± 5.673 36.1

LC-MS vs600.8320 ± 0.0236343.78 ± 11.4963.1

IMMULITE

LC-MS vs MSD1000.8310 ± 0.0317446.12 ± 12.6086.6

US11454637B2. Quantitation of insulin-like growth factor-I and insulin-like growth factor-II with high-resolution mass spectrometry (2022-09-27). Quest Diagnostics Investments Incorporated [US]. Inventors: Cory Bystrom [US], Shijun Sheng [US], Nigel Clarke [US], Richard E. Reitz [US].

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Estradiol Secretion Quantification

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3× 10⁵/well RL95-2 cells were seeded in 24-well-plates. After treated by 1 μM L-798,106 for 24 hours, the supernatant was collected and centrifuged (13,200 g, 10 min) to remove cell debris. The estradiol concentration was subsequently determined using chemiluminescent immunometric assay (IMMULITE 2000 immunoassay system) (Siemens, Germany) as described by the manufacturer.

Zhu J., Trillsch F., Mayr D., Kuhn C., Rahmeh M., Hofmann S., Vogel M., Mahner S., Jeschke U, & von Schönfeldt V. (2017). Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients. Oncotarget, 9(1), 982-994.

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Inflammatory Biomarkers in Serum Analysis

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Serum levels of CRP were determined following the manufacturer’s protocol using ABX Pentra 400 (Horiba Ltd., Kyoto, Japan). Serum samples were also used for the dosage of the following secreted inflammatory mediators according to the manufacturer’s protocol by Bioplex multiplex Human Cytokine, Chemokine and Growth factor kit (Bio-Rad, Hercules, CA, USA): IL-1β, IL-6, IL-10, IL-13, and TNFα. Serum samples were also assayed for cortisol and insulin levels by automated chemiluminescent immunoassay on the Immulite 2000 Immunoassay system (Siemens AG, Healthcare Sector, Erlangen, Germany).

Milone M., Desiderio A., Velotti N., Manigrasso M., Vertaldi S., Bracale U., D’Ambra M., Servillo G., De Simone G., De Palma F.D., Perruolo G., Raciti G.A., Miele C., Beguinot F, & De Palma G.D. (2021). Surgical stress and metabolic response after totally laparoscopic right colectomy. Scientific Reports, 11, 9652.

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Metabolic Markers in Diabetes Evaluation

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Plasma glucose levels were measured with use of the glucose oxidase method with a bedside glucose analyzer (Analox GM9D; Analox Instruments Ltd, Lunenberg, MA). Insulin, C-peptide, and human growth hormone were measured by a two-site chemiluminescent immunometric assay (IMMULITE 2000 immunoassay system; Siemens Healthcare Diagnostics Products Ltd, Llanberis, U.K.). Glucagon was measured using a competitive ELISA method employing a commerically available ELISA kit (ALPCO, Salem, NH). Plasma FFA levels were determined with an acyl-CoA oxidase method–based kit (Wako Diagnostics, Richmond, VA) on an automated modular analyzer (cobas 8000, Roche Diagnostics, Indianapolis, IN). Plasma glycerol levels were measured with a phosphorylation method–based calorimetric kit (Sigma-Aldrich, St. Louis, MO). Plasma lactate levels were estimated with enzymatic oxidation methods with commercial kits (cobas 8000). GAD-65 and IA-2 autoantibody positivity was analyzed with a quantitative sandwich immunoassay method (RSR Limited, Cardiff, U.K.) with an assay range of up to 5–2,000 units/mL for GAD-65 and up to 7.5–4,000 units/mL for IA-2.

Lontchi-Yimagou E., Dasgupta R., Anoop S., Kehlenbrink S., Koppaka S., Goyal A., Venkatesan P., Livingstone R., Ye K., Chapla A., Carey M., Jose A., Rebekah G., Wickramanayake A., Joseph M., Mathias P., Manavalan A., Kurian M.E., Inbakumari M., Christina F., Stein D., Thomas N, & Hawkins M. (2022). An Atypical Form of Diabetes Among Individuals With Low BMI. Diabetes Care, 45(6), 1428-1437.

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Serum IGF-1 and IGFBP-3 Measurement

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Blood samples were obtained at 8:00 a.m. following an overnight fast. Serum levels of IGF-1 and insulin-like growth factor binding protein 3 (IGFBP-3) were measured using routine laboratory assays (IMMULITE® 2000 immunoassay system (SIEMENS, Germany)). The z-score of IGF-1 was calculated using age- and sex-specific growth curves established in a previous study conducted by Cao et al [18 (link)].

Guo H., Fu J., Zhou Y., Luo F, & Cheng R. (2024). Evaluating the effect of recombinant human growth hormone treatment on sleep-related breathing disorders in toddlers with Prader–Willi syndrome: a one-year retrospective cohort study. BMC Pediatrics, 24, 32.

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Comprehensive Hormonal Assay Protocol

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Cortisol levels were measured by competitive electrochemiluminescent immunometric assay using the Cobas 8000 Modular Analyzer Series (Roche Diagnostics). For the cortisol assay, analytical sensitivity was 0.018 μg/dL and the intraassay coefficient of variation (CV) was 1.5%–1.7%. ACTH and DHEAS levels were measured by solid-phase two-site sequential and competitive chemiluminescent immunometric assays, respectively, using the IMMULITE 2000 Immunoassay System (Siemens Healthcare Diagnostics). For the ACTH assay, analytical sensitivity was 5 pg/mL, intraassay CV was 8.7%–9.5%, and interassay CV was 6.1%–10%. For the DHEAS assay, analytical sensitivity was 3 μg/dL, intraassay CV was 4.9%–9.8%, and interassay CV was 7.9%–13%. After urine extraction with dichloromethane, 24-h UFC was measured by the electrochemiluminescent technique with intra- and interassay CVs of 1.5%–1.7% and 1.8%–2.2%, respectively.

DURMUŞ E.T., ATMACA A., UZUNKAYA F., ÇOLAK R, & DURMUŞ B. (2022). The lateralization accuracy of inferior petrosal sinus sampling in Cushing’s disease: experiences of a tertiary center. Turkish Journal of Medical Sciences, 52(5), 1600-1608.

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Hormonal Ratio and Liver Enzyme Analysis

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They were measured in blood plasma using immunoassay analysers based on electrochemical luminescence detection technology. Prolactin was measured using the Immulite 2000 Immunoassay System (Siemens Healthcare GmbH, Erlangen, Germany) while testosterone was measured using the Cobas e 411 analyser (Roche Diagnostics GmbH, Mannheim, Germany). For each rat the prolactin to testosterone ratio was calculated. Activities of ALT and AST in the plasma were measured by COBAS INTEGRA 400 plus (Roche Diagnostic GmbH, Mannheim, Germany) automated analyser (Roche Diagnostics, Mannheim, Germany).

Nikolić-Kokić A., Tatalović N., Brkljačić J., Mijović M., Nestorović V., Mijušković A., Oreščanin-Dušić Z., Vidonja Uzelac T., Nikolić M., Spasić S., Blagojević D, & Miljević Č. (2022). Antipsychotic Drug-Mediated Adverse Effects on Rat Testicles May Be Caused by Altered Redox and Hormonal Homeostasis. International Journal of Molecular Sciences, 23(22), 13698.

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Intraoperative Parathyroid Hormone Monitoring

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Intraoperative serum parathyroid hormone (PTH) levels were obtained by peripheral venipuncture prior to incision and 15 minutes after excision of abnormal parathyroid gland(s). Testing was performed on an Immulite® 2000 Immunoassay System (Siemens Healthcare, Erlangen, Germany) with chemiluminescent immunoassay detection of intact PTH.

Goodsell K.E., Ermer J.P., Zaheer S., Kelz R.R., Fraker D.L, & Wachtel H. (2021). Double Adenoma as a Cause of Primary Hyperparathyroidism: Asymmetric Hyperplasia or a Distinct Pathologic Entity?. American journal of surgery, 222(3), 483-489.

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Diagnosing Diabetes and Hypothyroidism in Dogs

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Samples analysed for serum fructosamine were used for diagnosing DM, and analysis of TSH and FreeT4 in serum were used for diagnosing hypothyroidism based on defined criteria. The criterium for classifying a diabetic dog was a serum fructosamine concentration > 315 μmol/L. Fructosamine was analysed in serum by the Siemens Advia® 1800 Clinical Chemistry System (Siemens Healthcare GmbH, Germany) using the nitrobluetetrazolium-chloride (NBT) analytical method (Horiba Medical). For hypothyroidism, the criteria for classifying primary hypothyroidism in a dog were a serum TSH-concentration > 0.45 μg/L and a free thyroxine concentration (FT4) < 7 ρmol/L in the same sample. TSH and FT4 were analysed in serum by the Siemens Immulite® 2000 Immunoassay System using chemiluminescence methods (Siemens Healthcare GmbH, Germany). The laboratory has used the same analytical methods, analyzers and reference ranges during the whole period.

Ringstad N.K., Lingaas F, & Thoresen S.I. (2022). Breed distributions for diabetes mellitus and hypothyroidism in Norwegian dogs. Canine Medicine and Genetics, 9, 9.

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Hormonal and Bone Metabolism Markers

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Body mass index was calculated as weight (kg)/[height (m)] 2 . Serum levels of leptin, adiponectin, resistin, FGF23, a-Klotho, IGF1, GH, and osteocalcin were quantified using commercially available ELISA Research kits (Elabscience Biotechnology, USA). Serum parathyroid hormone (PTH) was quantified by electrochemiluminescence (ECLIA), using commercial kits (Advia Centaur Intact PTH Assay, Siemens Healthcare Diagnostics Inc., USA). Free T4, cortisol, oestradiol (in women), testosterone (in men), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were routinely measured in the Acro group via ECLIA (Immulite 2000 Immunoassay System, Siemens). Serum concentrations of calcium, phosphate, and glucose were determined by colorimetry (Cobas 6000 analyser, Roche). HbA1c was assessed via the ion-exchange high-performance liquid chromatography (HPLC) method. BMD at the lumbar spine (the mean BMD value for L1-L4 lumbar vertebrae), femoral neck, 1/3 radius, and whole-body levels were measured via DXA (Hologic Delphi A; Hologic Inc., USA) by 2 ho-

Bilha S.C., Matei A., Matei A., Constantinescu D., Pavel Tanasa M., Mogos-Cioncu R., Cianga P., Preda C, & Branisteanu D.D. (2021). New insights into the metabolic-bone crosstalk in active acromegaly. Endokrynologia Polska, 72(3).

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