H1299

Manufactured by Shanghai Cell Bank

Sourced in China

H1299 is a cell line derived from a human lung carcinoma. It is commonly used for in vitro research and experimentation purposes.

Automatically generated - may contain errors

Lab products found in correlation

52 protocols using h1299

Culturing Human Lung Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human normal bronchial epithelial cell line (BEAS-2B) and NSCLC cell lines (H1650, H1299,
A549, H1975, and HCC827) were obtained from the Shanghai Cell Bank of Chinese Academy of
Sciences (Shanghai, China). BEAS-2B cell line and NSCLC cells were maintained in RPMI1640
(Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand
Island, NY, USA), 100 μg/ml streptomycin, and 100 U/ml penicillin (Invitrogen, Carlsbad,
CA, USA) at 37˚C with 5% CO₂ .

Tan Q., Liu C., Shen Y, & Huang T. (2021). Circular RNA circ_0000517 Facilitates The Growth and Metastasis of Non-Small Cell Lung Cancer by Sponging miR-326/miR-330-5p. Cell Journal (Yakhteh), 23(5), 552-561.

+ Open protocol

+ Expand

NSCLC Cell Line Cultivation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human NSCLC cell lines A549, H460, H292, H1299, Calu1, and SK-MES-1 were obtained from the Shanghai Cell Bank (Shanghai, China). The HBE cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO₂.

Ren J., Liu T., Han Y., Wang Q., Chen Y., Li G, & Jiang L. (2018). GSK-3β inhibits autophagy and enhances radiosensitivity in non-small cell lung cancer. Diagnostic Pathology, 13, 33.

+ Open protocol

+ Expand

Lung Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human lung cancer cell lines, A549 and H1299, were obtained from the Shanghai Cell Bank (Shanghai, China) and authenticated by short tandem repeat DNA profiling. All cells were cultured in the Roswell Park Memorial Institute-1640 medium, 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich Co.) at 37°C and 5% CO₂ in a high humidity. VP (S1786) was purchased from Selleckchem (Shanghai, China).

Han Q., Kremerskothen J., Lin X., Zhang X., Rong X., Zhang D, & Wang E. (2018). WWC3 inhibits epithelial–mesenchymal transition of lung cancer by activating Hippo-YAP signaling. OncoTargets and therapy, 11, 2581-2591.

+ Open protocol

+ Expand

Culturing NSCLC Cell Lines A549 and H1299

Check if the same lab product or an alternative is used in the 5 most similar protocols

We purchased NSCLC cell lines A549 and H1299 from the Shanghai Cell Bank of the Chinese Academy of Sciences (China). The two cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% fetal bovine serum and placed in a constant temperature incubator at 37°C with 5% CO₂ and humidity saturation. The experiment was performed on cells in the logarithmic growth stage.

Ji J., Yang S., Zu L., Li Y, & Li Y. (2021). Deubiquitinating enzyme USP41 promotes lung cancer cell proliferation and migration. Thoracic Cancer, 12(7), 1041-1047.

+ Open protocol

+ Expand

Culturing Human NSCLC and Lung Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human NSCLC cell lines (A549, H1299, PC-9, HCC827, and H1975) and the normal human lung epithelial cell line BEAS-2B were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. All NSCLC cell lines were seeded in culture flasks and cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) at 37 °C in the presence of 95% O₂ and 5% CO₂. BEAS-2B cells were cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM, Gibco) under consistent culture conditions.

Wu B., Chang N., Xi H., Xiong J., Zhou Y., Wu Y., Wu S., Wang N., Yi H., Song Y., Chen L, & Zhang J. (2021). PHB2 promotes tumorigenesis via RACK1 in non-small cell lung cancer. Theranostics, 11(7), 3150-3166.

+ Open protocol

+ Expand

Cell Culture Protocols for Lung Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human bronchial epithelium (HBE) cell line was obtained from the American Type Culture Collection. The LK2 cell line was obtained from the Japanese Collection of Research Bioresources Cell Bank. The PG-LH7 (LH7) cell line was a gift from Dr Jie Zheng (Department of Pathology, Peking University). The A549, H1299, BE1, H292 and H460 cell lines were obtained from the Shanghai Cell Bank. All cells were cultured in RPMI-1640 (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Invitrogen; Thermo Fisher Scientific, Inc.), 100 IU/ml penicillin (Sigma-Aldrich; Merck KGaA), and 100 µg/ml streptomycin (Sigma-Aldrich; Merck KGaA) at 37˚C in 5% CO₂.

Liu X.F., Han Q., Rong X.Z., Yang M., Han Y.C., Yu J.H, & Lin X.Y. (2020). ANKHD1 promotes proliferation and invasion of non-small-cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway. International Journal of Oncology, 56(5), 1175-1185.

+ Open protocol

+ Expand

Cell Culture Protocols for NSCLC Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human bronchial epithelial (HBE) cell line was obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS). Human NSCLC cell lines (H1299, H460, H292 and A549) were obtained from the Shanghai Cell Bank (Shanghai, China) and cultured in RPMI1640 medium supplemented with 10% FBS (Gibco, NY, USA). All cell lines were cultured in 5% CO₂ at 37°C in a humidified incubator.

Sun S., Han Q., Liang M., Zhang Q., Zhang J, & Cao J. (2020). Downregulation of m6A reader YTHDC2 promotes tumor progression and predicts poor prognosis in non‐small cell lung cancer. Thoracic Cancer, 11(11), 3269-3279.

+ Open protocol

+ Expand

Culturing Lung Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human lung cancer cell lines A549, H460, H1299 and normal human bronchial epithelial cell line (HBE) were purchased from the Shanghai Cell Bank of Chinese Academy of Sciences. All cells were cultured in RPMI1640 cell culture medium containing 100 ml/L fetal bovine serum, 100 U/ml penicillin, 100 g/ml streptomycin and 2 mmol/L glutamine at 37°C with 5% CO₂ in full humidity.

Tang J., Li X., Cheng T, & Wu J. (2021). miR‐21‐5p/SMAD7 axis promotes the progress of lung cancer. Thoracic Cancer, 12(17), 2307-2313.

+ Open protocol

+ Expand

Culturing Diverse Lung Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HBE cell line was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The H1299, H460, A549, H292, and SK-MES-1 cell lines were purchased from the Shanghai Cell Bank (Shanghai, China). All of these cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA), 100 μg/ml streptomycin (Sigma, St Louis, MO, USA), and 100 IU/ml penicillin (Sigma, St Louis, MO, USA). Cells were passaged every other day using 0.25% trypsin (Invitrogen, Carlsbad, CA, USA).

Yu H., Sun J., Zhao C., Wang H., Liu Y., Xiong J., Chang J., Wang M., Wang W., Ye D., Zhou H, & Yu T. (2019). SET domain containing protein 5 (SETD5) enhances tumor cell invasion and is associated with a poor prognosis in non-small cell lung cancer patients. BMC Cancer, 19, 736.

+ Open protocol

+ Expand

Characterization of NSCLC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The HBE(human bronchial epithelioid cells)cell line was obtained from the American Type Culture Collection (ATCC); (Manassas, VA, USA). The LK2 cell line was a gift from H. Kijima (Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine, Japan). Other NSCLC cell lines, including A549, SPC, H1299, H292, H460 and H661, were purchased from the Shanghai Cell Bank (Shanghai, China). Under conditions of high humidity (37℃ and 5% CO₂), all cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA) supplemented with 10% foetal bovine serum (Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma‐Aldrich). All cell lines were identified using short tandem repeat DNA analysis.

Yang M., Han Y., Han Q., Rong X., Liu X, & Ln X. (2021). KCTD11 inhibits progression of lung cancer by binding to β‐catenin to regulate the activity of the Wnt and Hippo pathways. Journal of Cellular and Molecular Medicine, 25(19), 9411-9426.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!