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Berberine hydrochloride

Manufactured by Yuanye Bio-Technology
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Sourced in China

Berberine hydrochloride is a chemical compound that is used in laboratory settings. It is a crystalline powder with a yellow color. Berberine hydrochloride is a salt form of the natural alkaloid berberine.

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Lab products found in correlation

Acetonitrile, by Merck Group (2 mentions) Milli q purification system, by Merck Group (1 mentions) Metformin, by Selleck Chemicals (1 mentions) Fetal bovine serum (fbs), by Clark Bioscience (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) All in one mirna first strand cdna synthesis kit, by GeneCopoeia (1 mentions) Primerscript rt reagent kit with gdna eraser, by Takara Bio (1 mentions) Gapdh 5174, by Cell Signaling Technology (1 mentions) All in one mirna qpcr detection kit, by GeneCopoeia (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) Propranolol, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Beyotime (1 mentions) Ly294002, by Beyotime (1 mentions) Horseradish peroxidase labeled goat anti rabbit igg h l, by Beyotime (1 mentions) Palmatine hydrochloride, by Yuanye Bio-Technology (1 mentions)

5 protocols using berberine hydrochloride

1

Synthesis of Metal-Organic Framework

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Zirconium chloride (ZrCl4), 2-amino-1,4-benzenedicarboxylic acid (NH2–H2BDC), and PAD were purchased from McLean Reagent (Shanghai, China). Berberine hydrochloride was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. All chemicals were analytical grade or better, and the water used in all experiments was ultra-pure water.
Liu W., Wu S., Sun T.X., Bai J., Yang Y., Lian W.H, & Zhao Y. (2024). Post-synthetic modified luminescent metal–organic framework for the detection of berberine hydrochloride in a traditional Chinese herb. RSC Advances, 14(1), 602-607.
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2

Authenticated Herbal Compounds for In Vivo Study

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Coptidis Rhizoma (Coptis chinensis Franch.) and Glycyrrhizae Radix et Rhizoma (Glycyrrhiza uralensis Fisch.) were purchased from the Shanghai Kang Qiao Herbal Pieces Co., Ltd. (Shanghai, China), a GMP-certificated manufacturer without any affiliation to the authors. The herbs were authenticated by Prof. Zhi-Li Zhao of the Department of Botany, Shanghai University of Traditional Chinese Medicine, according to The Pharmacopoeia of People’s Republic of China (2015 edition). Berberine hydrochloride and carbamazepine with purities >98% (used as standards) were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Berberine hydrochloride with a purity >95% (used for oral administration in mice) was purchased from the Shanghai Yuan-Ye Biotechnology Co., Ltd. (Shanghai, China). Acetonitrile was purchased from Merck (Darmstadt, Germany). Ultra-pure water used in the current study was prepared using a Milli-Q purification system (Millipore Corporation, Billerica, MA, USA). All other materials were of analytical grade or higher.
Li Q., Yang Y., Zhou T., Wang R., Li N., Zheng M., Li Y.Y., Zhang J.Q., Wu F., Yang B.C., Ma Y.M, & Ma B.L. (2018). A Compositive Strategy to Study the Pharmacokinetics of TCMs: Taking Coptidis Rhizoma, and Coptidis Rhizoma-Glycyrrhizae Radix et Rhizoma as Examples. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(8), 2042.
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3

Exploring miRNA-Driven Molecular Mechanisms

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All-in-One™ miRNA first-strand cDNA synthesis kit, All-in-One™ miRNA qPCR detection kit and all quantitative (q)PCR primers were purchased from GeneCopoeia, Inc. Primerscript™ RT reagent kit with gDNA eraser and SYBR premix Ex Taq II were obtained from Takara Biotechnology Co., Ltd. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) was purchased from Beyotime Institute of Biotechnology. The cell culture media, including RPMI-1640 medium and DMEM, were obtained from Corning Inc. Fetal bovine serum (FBS) was purchased from Clark Bioscience. All primary antibodies, including those against E-cadherin (3195), β-catenin (8480), vimentin (5741), Twist1 (46702) and GAPDH (5174) were purchased from Cell Signaling Technology, Inc. The secondary antibody used was horseradish peroxidase-labeled goat anti-rabbit IgG (H+L; A0208) obtained from Beyotime Institute of Biotechnology. Berberine hydrochloride was purchased from Shanghai YuanYe Biotechnology Co., Ltd. Metformin and rapamycin were purchased from Selleck Chemicals. Propranolol was obtained from Sigma-Aldrich (Merck KGaA), LY294002 was ordered from Beyotime Institute of Biotechnology, and TOFA was purchased from Santa Cruz Biotechnology, Inc.
Chen Z., Liu Y., Qi B., Gu C., Wei X., Guo L., Yao W, & Zhao B. (2019). MicroRNA-212 facilitates the motility and invasiveness of esophageal squamous carcinoma cells. Molecular Medicine Reports, 20(4), 3633-3641.
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4

Analytical Characterization of Berberine

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The reference standard (purity, > 98%) of berberine hydrochloride was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). berberine hydrochloride (purity, > 95%) used for in vivo pharmacokinetic studies in mice was purchased from the Shanghai Yuan-Ye Biotechnology Co. Ltd. (Shanghai, China). The organic reagent acetonitrile was obtained from Merck (Darmstadt, Germany). All other materials were of analytical grade or higher.
Zhang J.Q., Wang R., Zhou T., Zhao Q., Zhao C.C, & Ma B.L. (2020). Pharmacokinetic incompatibility of the Huanglian-Gancao herb pair. BMC Complementary Medicine and Therapies, 20, 61.
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5

Evaluation of Antifungal Compounds

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Berberine hydrochloride (HPLC > 98 %, Lot Number: 20130306) and palmatine hydrochloride (HPLC > 98 %, Lot Number: 20130109) were purchased from Yuan Ye Biological Technology Co., Ltd, (Shanghai, China). Clotrimazole (99 % pure, Lot No. 23593-75-1) was purchased from BaDaTong Medical Company (TaiZhou, Zhejiang Province, China).
Xiao C.W., Ji Q.A., Wei Q., Liu Y, & Bao G.L. (2015). Antifungal activity of berberine hydrochloride and palmatine hydrochloride against Microsporum canis -induced dermatitis in rabbits and underlying mechanism. BMC Complementary and Alternative Medicine, 15, 177.
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