Pe labeled anti mouse cd11c

Manufactured by BioLegend

Sourced in United States

The PE-labeled anti-mouse CD11c is a flow cytometry reagent used to detect the CD11c marker on the surface of mouse cells. CD11c is a type I transmembrane glycoprotein and a member of the integrin alpha-X subfamily, which is expressed on the surface of dendritic cells, macrophages, and some natural killer cells.

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Lab products found in correlation

Fitc labeled anti mouse cd80, by BioLegend (2 mentions) Granulocyte macrophage colony stimulating factor, by Immunotools (1 mentions) Heat inactivated fetal bovine serum, by Lonza (1 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions) L glutamine, by Lonza (1 mentions) Dulbecco s modified eagle s medium, by Lonza (1 mentions) Flow cytometry, by BD (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Beyotime (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions) Mouse il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Pe labeled anti mouse mhcii, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-CD11c (97 citations) CD11c-PE (32 citations) PE anti-mouse CD11c (25 citations) Anti-CD11c-PE (20 citations) Anti-mouse CD11c (15 citations) PE-labeled anti-mouse CD11c antibody (3 citations) PE/Cy5-labeled anti-mouse CD11c (clone N418) hamster IgG (2 citations)

3 protocols using pe labeled anti mouse cd11c

Generation of mouse bone marrow-derived dendritic cells

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Bone marrow cells were isolated from the tibia of C57BL/6
mice and
cultured in Dulbecco’s modified Eagle’s medium (Lonza,
Basel, Switzerland) supplemented with 10% heat-inactivated fetal bovine
serum (Lonza), 2 mM l-glutamine (Lonza), 100 U/mL penicillin
(Lonza), 100 μg/mL streptomycin (Lonza), and 20 ng/mL granulocyte-macrophage
colony-stimulating factor (ImmunoTools, Friesoythe, Germany) for 7
days at 37 °C and 5% CO₂. The purity of the BMDCs
was evaluated with PE-labeled anti-mouse CD11c (Biolegend, San Diego,
CA, U.S.A.) by flow cytometry with >90% shown to be CD11c positive.

Guzelj S., Nabergoj S., Gobec M., Pajk S., Klančič V., Slütter B., Frkanec R., Štimac A., Šket P., Plavec J., Mlinarič-Raščan I, & Jakopin Ž. (2021). Structural Fine-Tuning of Desmuramylpeptide NOD2 Agonists Defines Their In Vivo Adjuvant Activity. Journal of Medicinal Chemistry, 64(11), 7809-7838.

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Quantifying Mature Dendritic Cells

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One week after the last vaccine dosing, splenocytes were isolated, red blood cells were lysed, and remaining cells were washed in PBS containing 1% bovine serum albumin at room temperature. Washed cells were stained for 30 min at room temperature with PE-labeled anti-mouse CD11c and FITC-labeled anti-mouse CD80 antibodies (Biolegend, San Diego, CA, USA). Double-positive cells considered to be mature dendritic cells were measured by flow cytometry (Becton Dickinson, San Jose, CA, USA).

Peng J., Ye L., Li T., Zhu Q., Guo J., Xiao K, & Wei Y. (2019). Irradiated Bladder Cancer Cells Expressing both GM-CSF and IL-21 versus Either GM-CSF or IL-21 Alone as Tumor Vaccine in a Mouse Xenograft Model. BioMed Research International, 2019, 8262989.

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Murine Bone Marrow-Derived Dendritic Cell Isolation and Activation

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Bone marrow cells were isolated from C57BL/6 mice and cultured in RPMI 1640 media (GIBCO) supplemented with 10% heat inactivated FBS (BioInd), 1% penicillin/streptomycin (Beyotime), 20 ng/mL of IL-4 (Peprotech)and 20 ng/mL of GM-CSF (Peprotech) at 37 C and 5% CO 2 . Non-adherent cells were harvested on day 7 and used immediately. DC purity was confirmed with PE labeled antimouse CD11c (Biolegend) by FACS, consistently found to be > 90% CD11c + .
In vitro BMDC stimulation Immature BMDCs (5 3 10 4 ) were planted onto 96 well plates, then treated with simvastatin (1 mM) or LPS (100 ng/mL) for 24 h. The cell supernatants IL-6 was measured by mouse IL-6 ELISA kit (Ebioscience), TNF-a was measured by mouse TNF-a ELISA kit (Ebioscience) and IL-12p70 was measured by mouse IL-12p70 ELISA kit (Ebioscience) according to the manufacturer's instructions. Cell-surface co-stimulatory molecules and MHC-II were detected by FITC labeled anti-mouse CD80 (Biolegend), anti-mouse CD86 (Biolegend) and PE labeled anti-mouse MHC-II (Ebioscience).

Xia Y., Xie Y., Yu Z., Xiao H., Jiang G., Zhou X., Yang Y., Li X., Zhao M., Li L., Zheng M., Han S., Zong Z., Meng X., Deng H., Ye H., Fa Y., Wu H., Oldfield E., Hu X., Liu W., Shi Y, & Zhang Y. (2018). The Mevalonate Pathway Is a Druggable Target for Vaccine Adjuvant Discovery. Cell, 175(4).

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