Log In Sign up
The largest database of trusted experimental protocols

Dnase 1 treatment

Manufactured by Bio-Rad
Visit Supplier

DNase I is an enzyme used to degrade DNA. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively removing DNA from samples.

Automatically generated - may contain errors

Lab products found in correlation

Quantstudio 5 real time pcr system, by Thermo Fisher Scientific (2 mentions) Cbot system, by Illumina (2 mentions) Truseq sr cluster kit v3, by Illumina (2 mentions) Truseq sbs kit, by Illumina (2 mentions) Nebnext ultra directional rna library prep kit, by New England Biolabs (2 mentions) Hiseq system, by Illumina (2 mentions) Nebnext poly a mrna magnetic isolation module, by New England Biolabs (2 mentions) Rnaqueous micro kit, by Thermo Fisher Scientific (2 mentions) Tissuelyser, by Qiagen (2 mentions) Iscript, by Bio-Rad (2 mentions) Bioanalyzer, by Agilent Technologies (2 mentions)

2 protocols using dnase 1 treatment

1

RNA-seq Library Preparation Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted using RNAqueous-micro kit (Thermo Fisher from frozen samples homogenized using TissueLyser (QIAGEN). cDNA was synthetized with the iScript after DNase I treatment (Biorad). The RNA quality was determined by Bioanalyzer (Agilent, Santa Clara, CA). To isolate the polyA RNA, NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used with a total of 1 μg of good quality total RNA as input. The NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs) using QuantStudio 5 Real-Time PCR Systems (Thermo Fisher). To study differential gene expression, individually indexed and compatible libraries were proportionally pooled (≥30 million reads per sample in general) for clustering in cBot system (Illumina, San Diego, CA). Libraries at the final concentration of 16.5 pM were clustered onto a single read (SR) flow cell using Illumina TruSeq SR Cluster kit v3, and sequenced to 51 bp using TruSeq SBS kit on Illumina HiSeq system (Sharma et al., 2018 (link)).
Holland J., Sorrell J., Yates E., Smith K., Arbabi S., Arnold M., Rivir M., Morano R., Chen J., Zhang X., Dimarchi R., Woods S.C., Sanchez-Gurmaches J., Wohleb E, & Perez-Tilve D. (2019). A Brain-Melanocortin-Vagus Axis Mediates Adipose Tissue Expansion Independently of Energy Intake. Cell reports, 27(8), 2399-2410.e6.
+ Open protocol
+ Expand
2

RNA-seq Library Preparation Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted using RNAqueous-micro kit (Thermo Fisher from frozen samples homogenized using TissueLyser (QIAGEN). cDNA was synthetized with the iScript after DNase I treatment (Biorad). The RNA quality was determined by Bioanalyzer (Agilent, Santa Clara, CA). To isolate the polyA RNA, NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used with a total of 1 μg of good quality total RNA as input. The NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs) using QuantStudio 5 Real-Time PCR Systems (Thermo Fisher). To study differential gene expression, individually indexed and compatible libraries were proportionally pooled (≥30 million reads per sample in general) for clustering in cBot system (Illumina, San Diego, CA). Libraries at the final concentration of 16.5 pM were clustered onto a single read (SR) flow cell using Illumina TruSeq SR Cluster kit v3, and sequenced to 51 bp using TruSeq SBS kit on Illumina HiSeq system (Sharma et al., 2018 (link)).
Holland J., Sorrell J., Yates E., Smith K., Arbabi S., Arnold M., Rivir M., Morano R., Chen J., Zhang X., Dimarchi R., Woods S.C., Sanchez-Gurmaches J., Wohleb E, & Perez-Tilve D. (2019). A Brain-Melanocortin-Vagus Axis Mediates Adipose Tissue Expansion Independently of Energy Intake. Cell reports, 27(8), 2399-2410.e6.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!