The cembranoid 4R was prepared by Dr. K. El Sayed (School of Pharmacy, University of Louisiana, Monroe, LA) as previously described [30 ]. The purity of the batch used for these experiments was more than 98%. The 4R solvent consisted of 99.6% polyethylene glycol (PEG) and 0.4% dimethyl sulfoxide (DMSO). LPS from Escherichia coli serotype 026:B6 was purchased from Sigma-Aldrich (St. Louis, MO) and freshly dissolved in sterile saline (0.9% NaCl). N-Methyl-d -aspartic acid (NMDA) and dihydro-β-erythroidine (DHβE) were purchased from Sigma-Aldrich. Methyllycaconitine (MLA) was purchased from Calbiochem (La Jolla, CA).
Dihydro β erythroidine dhβe
Manufactured by Merck Group
Sourced in United States
Dihydro-β-erythroidine (DHβE) is a laboratory reagent used in research applications. It functions as a selective antagonist for nicotinic acetylcholine receptors. The core purpose of this compound is to facilitate the study of nicotinic receptor physiology and pharmacology in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Picrotoxin, by Merck Group (2 mentions)
Qx 314, by Bio-Techne (2 mentions)
Sr16584, by Bio-Techne (2 mentions)
Acetylcholine ach, by Merck Group (2 mentions)
Rubi nicotine, by Abcam (2 mentions)
α conotoxin mii α ctxmii, by Merck Group (2 mentions)
D ap5, by Bio-Techne (2 mentions)
Lps from escherichia coli serotype 026 b6, by Merck Group (1 mentions)
N methyl d aspartic acid nmda, by Merck Group (1 mentions)
Epibatidine, by Merck Group (1 mentions)
Mecamylamine mec, by Merck Group (1 mentions)
Cell tak, by Merck Group (1 mentions)
8 cpt camp, by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Saclofen, by Bio-Techne (1 mentions)
Bicuculline, by Bio-Techne (1 mentions)
Oxotremorine m oxo m, by Bio-Techne (1 mentions)
7 protocols using dihydro β erythroidine dhβe
1
Cembranoid 4R Preparation and Characterization
2
Electrophysiological Assay for Cholinergic Compounds
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Acetylcholine (ACh), imidacloprid, and dihydro-β-erythroidine (DHβE) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cycloxaprid was synthesized and purified, as previously reported [12 (link)]. In the electrophysiological experiments, chemical solutions were freshly prepared in the modified Ringer’s solution (NaCl 150 mM, KCl 2.8 mM, HEPES 10 mM, MgCl2 2 mM, atropine sulfate 0.5 µM; pH 7.2, adjusted with NaOH).
3
Neurotransmitter Receptor Ligand Acquisition
4
Pharmacological Modulation of Nicotinic Receptors
5
Nicotine-free Receptor Pharmacology
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(-) Nicotine free base (NFB) ((−)-1-Methyl-2-(3-pyridyl)pyrrolidine, (S)-3-(1-Methyl-2-pyrrolidinyl)pyridine), ethanol, acetylcholine, 8-(4-Chlorophenylthio) adenosine 3',5'-cyclic monophosphate (8-CPT-cAMP), 1,2- Bis(2- aminophenoxy) ethane- N,N,N′,N′-tetraacetic acid tetrakis-(acetoxymethyl ester) (BAPTA-AM), ionomycin, H89 (N-[2-[[3-(4-Bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride), mecamylamine (Mec), dihydro-β-erythroidine (DHβE), HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), and CellTak were obtained from Sigma-Aldrich. CP-601932, a partial agonists of α3β4* nAChR, was obtained from Pfizer Inc. (* indicates the possibility of additional subunits).
6
Pharmacological Profiling of Receptor Interactions
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D-APV, GYKI 52466 hydrochloride (GYKI), oxotremorine-M (Oxo-M), MCPG, bicuculline and saclofen were obtained from Tocris Bioscience or Ascent Scientific. Dihydro-β-erythroidine (DHβE) and other chemicals were obtained from Sigma Aldrich. Stock aliquots of drugs were prepared at 1000–10 000× final concentrations in de-ionized water or aqueous acid (GYKI) and stored at −20°C. None of the drugs applied altered electrode sensitivity to DA. Data from immediately prior to drug application were compared with data acquired when drug effects equilibrated, which was typically 10–20 min after application.
7
Pharmacological Modulation of Nicotinic Receptors
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QX-314, CNQX, SR16584, and D-AP5 were obtained from Tocris.
RuBi-nicotine was obtained from Abcam. α-conotoxin MII (α-Ctx
MII) was synthesized as previously described30 (link),31 (link).
Acetylcholine (ACh), mecamylamine (mec), atropine, picrotoxin,
dihydro-β-erythroidine (DHβE), and all other chemicals without a
specified supplier were obtained from Sigma.
RuBi-nicotine was obtained from Abcam. α-conotoxin MII (α-Ctx
MII) was synthesized as previously described30 (link),31 (link).
Acetylcholine (ACh), mecamylamine (mec), atropine, picrotoxin,
dihydro-β-erythroidine (DHβE), and all other chemicals without a
specified supplier were obtained from Sigma.
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