Considering that Nischarin is a target of antihypertensive drugs, we investigated whether such drugs could affect NISCH expression and the abnormal murine behaviors caused by the dysregulation of this gene. About 50,000 U251 cells were plated into 24-well plates, and were treated with either DMSO or 0.1 mM clonidine (#C7897, Sigma-Aldrich) or 0.1 mM tizanidine (#T6950, Sigma-Aldrich) on the second day for 72 h. Total RNA was then harvested, reverse-transcribed into cDNA, and the expression level of NISCH was determined by RT-qPCR. The mice were fed with water containing either DMSO or 0.1 mM clonidine for 72 h before the behavioral experiments.
Tizanidine
Manufactured by Merck Group
Sourced in United States
Tizanidine is a laboratory equipment product manufactured by Merck Group. It is a centrally acting muscle relaxant used to reduce muscle spasticity. The core function of Tizanidine is to serve as a tool for research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using tizanidine
1
Antihypertensive Drugs Modulate Nischarin Expression
2
Intrathecal Drug Injection in Rats
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Tizanidine (2.5 µg/20 µl), PDTC (2 nmol/20 µl) and BRL44408 (15 µg/20 µl; Sigma, St. Louis, MO, USA) were diluted in normal saline (NS, 0.9% NaCl) and loaded into 12.7 mm 30 gauge needle. Under inhalation anaesthesia using isoflurane (2% in oxygen), the rats were injected with drug at the L5-6 interspace.
3
Spasticity Treatment Evaluation in Rats
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Spastic rats at 3 months post-transection were implanted with intraperitoneal PE-10 catheter for drug delivery. Three days after catheter implant the baseline spasticity response was measured using ankle-rotational system. Animals were the injected with either Baclofen (Sigma, 10 mg/kg), Tizanidine (Sigma, 1 mg/kg), or NGX424 (Tezampanel, TorreyPines therapeutics, Inc., 1 mg/kg) delivered as a single bolus injection over 60 sec interval. After treatment the anti-spasticity effect was measured in 5 minute intervals for two hours. In a separate recording sessions the effect of identical treatments on the H-reflex and tactile stimulus-evoked hyper-reflexia was tested at 1 hr post-injection.
4
GPCR-Targeted Drug Activation Assay
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Time-series data obtained from calcium imaging for a set of GPCR-targeting drugs were used in the analysis. SDF-1α (Sigma, St. Louis, MO; Cayman Chemical, Ann Arbor, MI) in Hank's balanced salt solution (HBSS) was used to activate the CXCR4 receptor at different concentrations (1 -400 ng/mL). Also, histamine (0.001-1 µM) and endothelin (0.05-1 µM) (Sigma) were used to activate histamine and endothelin receptors respectively. A set of drugs having various chemotypes, namely, norepinephrine, tizanidine, clonidine, and dexmeditomidine (Sigma, Cayman Chemical), in HBSS were used to activate α2AR at different concentrations (0.1 µM-1 mM). For drug exposure, the dishes with cells were randomly selected in an unbiased manner.
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